Modulation of nucleotide pyrophosphatase in plasmacytoma cells

Neil F. Rebbe, Scot Hickman

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The effect of glucocorticoid hormones on the protein responsible for both nucleotide pyrophosphatase (EC 3.6.1.9) and alkaline phosphodiesterase I (EC 3.1.4.1) activities was examined in murine MOPC 315 plasmacytoma cells. Incubation of these cells with dexamethasone resulted in parallel increases in pyrophosphatase and phosphodiesterase specific activities. The incorporation of [3H]mannose into N-linked oligosaccharide precursors was also analyzed in cells following hormone modulation. In cells treated for 36 hours or cultured continuously with dexamethasone, the resulting increase in enzyme specific activities was accompanied by a decrease in [3H]mannose incorporation, consistent with the hypothesis that in some cell types, nucleotide pyrophosphatase activity is involved in the regulation of glycoprotein synthesis.

Original languageEnglish
Pages (from-to)637-644
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume175
Issue number2
DOIs
StatePublished - Mar 15 1991

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