Modulation of nicotinic ACh-, GABA(A)- and 5-HT3-receptor functions by external H-7, a protein kinase inhibitor, in rat sensory neurones

Hong Zhen Hu, Zhi Wang Li

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14 Scopus citations

Abstract

1. The effects of external H-7, a potent protein kinase inhibitor, on the responses mediated by γ-aminobutyric acid A type (GAGA(A))-, nicotinic acetylcholine (nicotinic ACh)-, ionotropic 5-hydroxytryptamine (5-HT3)-, adenosine 5'-triphosphate (ATP)-, N-methyl-D-aspartate (NMDA)- and kainate (KA)-receptors were studied in freshly dissociated rat dorsal root ganglion neurone by use of whole cell patch-clamp technique. 2. External H-7 (1-1000 μM) produced a reversible, dose-dependent inhibition of whole cell currents activated by GABA, ACh and 5-HT. 3. Whole-cell currents evoked by ATP, 2-methylthio-ATP, NMDA and KA were sensitive to external H-7. 4. External H-7 shifted the dose-response curve of GABA-activated currents downward without changing the EC50 significantly (from 15.0 ± 4.0 μM to 18.0 ± 5.0 μM). The maximum response to GABA was depressed by 34.0 ± 5.3%. This inhibitory action of H-7 was voltage-independent. 5. Intracellular application of H-7 (20 μM), cyclic AMP (1 mM) and BAPTA (10 mM) could not reverse the H-7 inhibition of GABA-activated currents. 6. The results suggest that external H-7 selectively and allosterically modulates the functions of GABA(A)-, nicotine ACh- and 5-HT3 receptors via a common conserved site in the external domain of these receptors.

Original languageEnglish
Pages (from-to)1195-1201
Number of pages7
JournalBritish Journal of Pharmacology
Volume122
Issue number6
DOIs
StatePublished - 1997

Keywords

  • Dorsal root ganglion
  • Glutamate receptors
  • H-7
  • Modulation
  • Nicotinic receptor superfamily
  • P2X receptor
  • Whole cell patch clamp recording

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