Modified western blot assay for confirmation and differentiation of human T cell lymphotropic virus types I and II

J. J. Lipka, P. Santiago, L. Chan, G. R. Reyes, K. P. Samuel, W. A. Blattner, G. M. Shaw, C. V. Hanson, J. J. Sninsky, S. K.H. Foung

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31 Scopus citations

Abstract

Disease association studies of human T cell lymphotropic virus (HTLV) types I and II are hindered by the need for multiple assays to confirm and differentiate between the viruses. A modified Western blot assay has been developed using HTLV-I viral lysate and unique (MTA-4) and shared (p21E) HTLV recombinant proteins. By defining confirmation of infection as the presence of antibodies to p24 gag protein and to p21E, all 56 HTLV-I and 49 HTLV-II antisera were confirmed by this modified Western blot alone. Differentiation was determined by reactivity to MTA-4. All HTLV-I antisera reacted with MTA-4 and all HTLV-II antisera did not react with MTA-4. These findings indicate the utility of selected HTLV-I recombinant proteins in a single assay format to confirm and differentiate infections with HTLV-I and HTLV-II.

Original languageEnglish
Pages (from-to)400-403
Number of pages4
JournalJournal of Infectious Diseases
Volume164
Issue number2
DOIs
StatePublished - Aug 1991

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