Modified high-performance liquid chromatography with electrochemical detection method for plasma measurement of levodopa, 3-O-methyldopa, dopamine, carbidopa and 3,4-dihydroxyphenyl acetic acid

Morvarid Karimi, Juanita L. Carl, Susan Loftin, Joel S. Perlmutter

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

Plasma measurements of levodopa and its major metabolites including dopamine and 3-O-methyldopa have been limited by cumbersome methods and poor sensitivity within relatively narrow ranges of plasma levels. We now report a modification of an HPLC method that permits concomitant measurements of a wide range of concentrations of levodopa, dopamine (DA), carbidopa, 3-O-methyldopa (3-OMD) and 3,4-dihydroxyphenyl acetic acid (DOPAC) from one HPLC injection. The recoveries ranged from 77 to 107% with an intra-day precision around 5% (CV) and inter-day CV's about 10-20%. This validated method will simplify pharmacokinetic studies of levodopa and its metabolites for mechanistic studies or therapeutic clinical monitoring which play a crucial role in development of strategies to prolong motor benefits from individual doses and reduce involuntary movements called dykinesias.

Original languageEnglish
Pages (from-to)120-123
Number of pages4
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume836
Issue number1-2
DOIs
StatePublished - May 19 2006

Keywords

  • 3-O-Methyldopa
  • Carbidopa
  • DOPAC (3,4-dihydroxyphenyl acetic acid)
  • Dopamine
  • HPLC
  • Levodopa
  • Plasma measurements

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