Modification of the immobilized metal affinity electrophoresis using sodium dodecyl sulfate polyacrylamide gel electrophoresis

Bao Shiang Lee, G. D.Lasanthi P. Jayathilaka, Jin Sheng Huang, David Decresce, Jeffrey A. Borgia, Xiaofeng Zhou, Shalini Gupta

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Modification to the original immobilized metal affinity electrophoresis (IMAEP) technique is presented. SDS-PAGE is used instead of native PAGE for improved extraction of phosphoproteins from a mixture of proteins. Protein samples treated with 2% w/v SDS instead of native sample buffer ensure that proteins are negatively charged. These negative charges on the proteins assure that the proteins migrate electrophoretically towards the anode regardless of their pI values and hence pass through the region embedded with the metal ions. Another benefit of treating proteins with SDS is that it unfolds the phosphoproteins exposing the phosphate groups to facilitate the metal-phosphate interactions. Phosphorylated ovalbumin can only be extracted after SDS sample buffer treatment. Data show that there is no detrimental effect upon SDS treatment on the extraction of phosphoproteins from a mixture of proteins. Electrophoretic migration of phosphoproteins ceases upon encounter with metal ions like Al+3, Ti+3, Fe+3, Fe+2 , and Mn+2 whereas non-phosphorylated proteins migrate freely.

Original languageEnglish
Pages (from-to)3160-3163
Number of pages4
JournalElectrophoresis
Volume29
Issue number15
DOIs
StatePublished - Aug 1 2008
Externally publishedYes

Keywords

  • Immobilized metal affinity column
  • Immobilized metal affinity electrophoresis
  • Phosphoproteins
  • Polyacrylamide gel
  • Sodium dodecyl sulfate

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