Modification of a trypsin‐detergent method for DNA flow cytometry of human epidermis

Dowling B. Stough; E. Robert Burns; Susan B. Mallory; James L. Pipkin; William G. Hinson

doi:10.1002/cyto.990100116

Modification of a trypsin‐detergent method for DNA flow cytometry of human epidermis

Dowling B. Stough, E. Robert Burns, Susan B. Mallory, James L. Pipkin, William G. Hinson

Division of Dermatology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

An existing technique (Vindelov et al. Cytometry 3:323, 1983) has been modified for DNA flow cytometry of human epidermis obtained from 2 to 3 mm punch biopsies. By varying the length of time of digestion of the epidermal disc by trypsin from 5 to 70 min a controlled release of keratinocytes occurred beginning with the stratum basale and proceeding toward, but not including, the superficial layer of the epidermis, the stratum corneum.

Original language	English
Pages (from-to)	90-93
Number of pages	4
Journal	Cytometry
Volume	10
Issue number	1
DOIs	https://doi.org/10.1002/cyto.990100116
State	Published - Jan 1989

Keywords

Trypsin digestion
biopsy
isolated nuclei
keratinocyte

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abstract = "An existing technique (Vindelov et al. Cytometry 3:323, 1983) has been modified for DNA flow cytometry of human epidermis obtained from 2 to 3 mm punch biopsies. By varying the length of time of digestion of the epidermal disc by trypsin from 5 to 70 min a controlled release of keratinocytes occurred beginning with the stratum basale and proceeding toward, but not including, the superficial layer of the epidermis, the stratum corneum.",

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AU - Hinson, William G.

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N2 - An existing technique (Vindelov et al. Cytometry 3:323, 1983) has been modified for DNA flow cytometry of human epidermis obtained from 2 to 3 mm punch biopsies. By varying the length of time of digestion of the epidermal disc by trypsin from 5 to 70 min a controlled release of keratinocytes occurred beginning with the stratum basale and proceeding toward, but not including, the superficial layer of the epidermis, the stratum corneum.

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KW - biopsy

KW - isolated nuclei

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Modification of a trypsin‐detergent method for DNA flow cytometry of human epidermis

Abstract

Keywords

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