TY - JOUR
T1 - Modern Laser Scanning Confocal Microscopy
AU - Bayguinov, Peter O.
AU - Oakley, Dennis M.
AU - Shih, Chien Cheng
AU - Geanon, Daniel J.
AU - Joens, Matthew S.
AU - Fitzpatrick, James A.J.
PY - 2018/7
Y1 - 2018/7
N2 - Since its commercialization in the late 1980's, confocal laser scanning microscopy (CLSM) has since become one of the most prevalent fluorescence microscopy techniques for three-dimensional structural studies of biological cells and tissues. The flexibility of the approach has enabled its application in a diverse array of studies, from the fast imaging of dynamic processes in living cells, to meticulous morphological analyses of tissues, and co-localization of protein expression patterns. In this chapter, we introduce the principles of confocal microscopy and discuss how the approach has become a mainstay in the biological sciences. We describe the components of a CLSM system and assess how modern implementations of the approach have further expanded the use of the technique. Finally, we briefly outline some practical considerations to take into account when acquiring data using a CLSM system.
AB - Since its commercialization in the late 1980's, confocal laser scanning microscopy (CLSM) has since become one of the most prevalent fluorescence microscopy techniques for three-dimensional structural studies of biological cells and tissues. The flexibility of the approach has enabled its application in a diverse array of studies, from the fast imaging of dynamic processes in living cells, to meticulous morphological analyses of tissues, and co-localization of protein expression patterns. In this chapter, we introduce the principles of confocal microscopy and discuss how the approach has become a mainstay in the biological sciences. We describe the components of a CLSM system and assess how modern implementations of the approach have further expanded the use of the technique. Finally, we briefly outline some practical considerations to take into account when acquiring data using a CLSM system.
KW - confocal laser scanning microscopy (CLSM)
KW - fluorescent imaging
KW - live-cell imaging
KW - optical sectioning
KW - spinning disk confocal
KW - swept-field confocal
KW - three-dimensional imaging
UR - http://www.scopus.com/inward/record.url?scp=85049772946&partnerID=8YFLogxK
U2 - 10.1002/cpcy.39
DO - 10.1002/cpcy.39
M3 - Comment/debate
C2 - 29927100
AN - SCOPUS:85049772946
VL - 85
JO - Current Protocols in Cytometry
JF - Current Protocols in Cytometry
SN - 1934-9297
IS - 1
M1 - e39
ER -