Modern approaches for investigating signaling pathways

Adam G. Evertts, Barry M. Zee, Benjamin A. Garcia

Research output: Contribution to journalReview articlepeer-review

27 Scopus citations

Abstract

Epigenetics is increasingly being recognized as a central component of physiological processes as diverse as obesity and circadian rhythms. Primarily acting through DNA methylation and histone posttranslational modifications, epigenetic pathways enable both short-and long-term transcriptional activation and silencing, independently of the underlying genetic sequence. To more quantitatively study the molecular basis of epigenetic regulation in physiological processes, the present review informs the latest techniques to identify and compare novel DNA methylation marks and combinatorial histone modifications across different experimental conditions, and to localize both DNA methylation and histone modifications over specific genomic regions.

Original languageEnglish
Pages (from-to)927-933
Number of pages7
JournalJournal of Applied Physiology
Volume109
Issue number3
DOIs
StatePublished - Sep 2010

Keywords

  • ChIP-SEQ
  • DNA methylation
  • Histone posttranslational modifications
  • Mass spectrometry

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