TY - JOUR
T1 - Modeling oxidative stress in the central nervous system
AU - Lehtinen, Maria K.
AU - Bonni, Azad
PY - 2006/12
Y1 - 2006/12
N2 - Oxidative stress is associated with the onset and pathogenesis of several prominent central nervous system disorders. Consequently, there is a pressing need for experimental methods for studying neuronal responses to oxidative stress. A number of techniques for modeling oxidative stress have been developed, including the use of inhibitors of the mitochondrial respiratory chain, depletion of endogenous antioxidants, application of products of lipid peroxidation, use of heavy metals, and models of ischemic brain injury. These experimental approaches can be applied from cell culture to in vivo animal models. Their use has provided insight into the molecular underpinnings of oxidative stress responses in the nervous system, including cell recovery and cell death. Reactive oxygen species contribute to conformational change-induced activation of signaling pathways, inactivation of enzymes through modification of catalytic cysteine residues, and subcellular redistribution of signaling molecules. In this review, we will discuss several methods for inducing oxidative stress in the nervous system and explore newly emerging concepts in oxidative stress signaling.
AB - Oxidative stress is associated with the onset and pathogenesis of several prominent central nervous system disorders. Consequently, there is a pressing need for experimental methods for studying neuronal responses to oxidative stress. A number of techniques for modeling oxidative stress have been developed, including the use of inhibitors of the mitochondrial respiratory chain, depletion of endogenous antioxidants, application of products of lipid peroxidation, use of heavy metals, and models of ischemic brain injury. These experimental approaches can be applied from cell culture to in vivo animal models. Their use has provided insight into the molecular underpinnings of oxidative stress responses in the nervous system, including cell recovery and cell death. Reactive oxygen species contribute to conformational change-induced activation of signaling pathways, inactivation of enzymes through modification of catalytic cysteine residues, and subcellular redistribution of signaling molecules. In this review, we will discuss several methods for inducing oxidative stress in the nervous system and explore newly emerging concepts in oxidative stress signaling.
KW - Apoptosis inducing factor
KW - Coenzyme Q
KW - Harlequin
KW - Neuronal survival
KW - Pheochromocytoma cell line
UR - http://www.scopus.com/inward/record.url?scp=33845401465&partnerID=8YFLogxK
U2 - 10.2174/156652406779010786
DO - 10.2174/156652406779010786
M3 - Review article
C2 - 17168738
AN - SCOPUS:33845401465
VL - 6
SP - 871
EP - 881
JO - Current Molecular Medicine
JF - Current Molecular Medicine
SN - 1566-5240
IS - 8
ER -