Modeling of the complex between transducin and photoactivated rhodopsin, a prototypical G-protein-coupled receptor

Gregory V. Nikiforovich, Christina M. Taylor, Garland R. Marshall

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Obtaining a reliable 3D model for the complex formed by photoactivated rhodopsin (R*) and its G-protein, transducin (Gtαβγ), would significantly benefit the entire field of structural biology of G-protein-coupled receptors (GPCRs). In this study, we have performed extensive configurational sampling for the isolated C-terminal fragment of the α-subunit of transducin, Gtα 340-350, within cavities of photoactivated rhodopsin formed by different energetically feasible conformations of the intracellular loops. Our results suggested a new 3D model of the rhodopsin-transducin complex that fully satisfied all available experimental data on site-directed mutagenesis of rhodopsin and Gtαβγ as well as data from disulfide-linking experiments. Importantly, the experimental data were not used as a priori constraints in model building. We performed a thorough comparison of existing computational models of the rhodopsin-transducin complex with each other and with current experimental data. It was found that different models suggest interactions with different molecules in the rhodopsin oligomer, that providing valuable guidance in design of specific novel experimental studies of the R*- Gtαβγ complex. Finally, we demonstrated that the isolated Gtα 340-350 fragment does not necessarily bind rhodopsin in the same binding mode as the same segment in intact Gtα.

Original languageEnglish
Pages (from-to)4734-4744
Number of pages11
JournalBiochemistry
Volume46
Issue number16
DOIs
StatePublished - Apr 24 2007

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