Modeling molecular and cellular aspects of human disease using the nematode Caenorhabditis elegans

Gary A. Silverman, Cliff J. Luke, Sangeeta R. Bhatia, Olivia S. Long, Anne C. Vetica, David H. Perlmutter, Stephen C. Pak

Research output: Contribution to journalReview article

57 Scopus citations

Abstract

As an experimental system, Caenorhabditis elegans offers a unique opportunity to interrogate in vivo the genetic and molecular functions of human disease-related genes. For example, C. elegans has provided crucial insights into fundamental biologic processes, such as cell death and cell fate determinations, as well as pathologic processes such as neurodegeneration and microbial susceptibility. The C. elegans model has several distinct advantages, including a completely sequenced genome that shares extensive homology with that of mammals, ease of cultivation and storage, a relatively short lifespan and techniques for generating null and transgenic animals. However, the ability to conduct unbiased forward and reverse genetic screens in C. elegans remains one of the most powerful experimental paradigms for discovering the biochemical pathways underlying human disease phenotypes. The identification of these pathways leads to a better understanding of the molecular interactions that perturb cellular physiology, and forms the foundation for designing mechanism-based therapies. To this end, the ability to process large numbers of isogenic animals through automated work stations suggests that C. elegans, manifesting different aspects of human disease phenotypes, will become the platform of choice for in vivo drug discovery and target validation using high-throughput/content screening technologies.

Original languageEnglish
Pages (from-to)10-18
Number of pages9
JournalPediatric research
Volume65
Issue number1
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

Fingerprint Dive into the research topics of 'Modeling molecular and cellular aspects of human disease using the nematode Caenorhabditis elegans'. Together they form a unique fingerprint.

  • Cite this