Mobile group II intron targeting: Applications in prokaryotes and perspectives in eukaryotes

Xiaoxia Cui, Greg Davis

Research output: Contribution to journalReview articlepeer-review

14 Scopus citations

Abstract

Mobile group II introns are ribozymes and use a novel mechanism-target DNA-primed reverse transcription-to proliferate in DNA. Group II introns are a unique mobile element for their high sequencespecific, yet readily flexible target site recognition. Both the intron RNA and the intron-encoded protein (IEP) are involved in target site recognition, and the specificity is determined primarily by base pairing between the intron RNA and DNA target. Therefore, the intron RNA can be modified according to the desired target sequence for specific gene disruption. Group II intron knockout technology is mature in bacteria and is currently being developed in eukaryotes. This technology has great potential to revolutionize fields such as functional genomics, gene therapy, and cell line engineering.

Original languageEnglish
Pages (from-to)4972-4985
Number of pages14
JournalFrontiers in Bioscience
Volume12
Issue number13
DOIs
StatePublished - Sep 1 2007

Keywords

  • Cell line engineering
  • Double-strand break
  • Functional genomics
  • Gene knockout
  • Gene targeting
  • Gene therapy
  • Homologous recombination
  • Reverse splicing
  • Reverse transcriptase
  • Reverse transcription
  • Review
  • Ribozyme
  • Splicing
  • Targetron
  • Transposons

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