Mitogen-stimulated phospholipid synthesis in normal and immune-deficient human B cells

M. M. Chien, W. M. Yokoyama, R. F. Ashman

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Eight patients with common variable panhypogammaglobulinemia were shown in the in vitro Ig biosynthesis assay to have defective B cell responses to pokeweed mitogen (PWM). Phospholipid synthesis was assessed in the B cell plus monocyte fraction (MB) and irradiated T cells (T*) of patients and paired normal controls. Cell populations were studied separately and in the four possible combinations (1:1), with and without PWM, to reveal the effect of cell interactions. At 16 to 20 hr the mean stimulation index (SI) ± standard error for MB cells alone was 1.01 ± 0.02 for eight patients and 0.99 ± 0.02 for the paired normals; the T* cell SI was 1.25 ± 0.04 for patients and 1.28 ± 0.05 for normals. Combinations of normal MB cells with normal T* cells showed significantly higher SI when compared with the combinations of normal MB cells with patient T* cells (p < 0.005). However, the combination of patient MB cells with patient T* cells and the combination of patient MB cells with normal T* cells were not significantly different in SI (0.05 < p < 0.1). Isolation of patient and normal B cells, T* cells, and monocytes after the choline pulse showed that patient B cells gave a higher SI with normal T* help than with patient T* help. Of greatest interest is the finding that patient B cells that were defective in PWM-stimulated Ig production nevertheless showed a phospholipid synthesis response to PWM in the normal range, suggesting that the maturation defect in these B cells occurs later than the phospholipid synthesis acceleration step, or on a different pathway.

Original languageEnglish
Pages (from-to)2844-2850
Number of pages7
JournalJournal of Immunology
Volume136
Issue number8
StatePublished - Jan 1 1986
Externally publishedYes

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