Mitochondrial metabolism underlies hyperoxic cell damage

Jian Li, Xueshan Gao, Mingwei Qian, John W. Eaton

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

Exposure of mammals to hyperoxia causes pulmonary and ocular pathology. Hyperoxic damage and cell death may derive from enhanced intracellular formation of reactive oxygen species (ROS), probably of mitochondrial origin. There is, however, controversy on this point. When wild-type and respiration-deficient (ρ°) HeLa cells were cultured in 80% O2, wild-type cells stopped growing after 5 days and died thereafter whereas ρ°cells survived and grew to confluence. This tolerance of ρ°cells for hyperoxia was not associated with greater resistance to oxidants such as hydrogen peroxide and t-butyl hydroperoxide. Under both 20% and 80% O2, ρ°cells exhibited substantially decreased ROS production, and, under 80% O2, ρ°cells showed no suppression of aconitase activity or mitochondrial protein carbonyl formation. Replacement of normal mitochondria in ρ°cells restored ROS production and susceptibility to hyperoxia. Two other approaches that diminished mitochondrial ROS generation also increased tolerance for hyperoxia. HeLa cells constantly exposed to the protonophoric uncoupler carbonyl cyanide m-chlorophenylhydrazone, which enhances respiration but decreases ROS production, showed preferential survival under 80% O 2, as did HeLa cells treated with chloramphenicol, which suppresses both respiration and mitochondrial ROS production. We conclude that interactions between respiring mitochondria and O2 are primarily responsible for hyperoxic cell damage.

Original languageEnglish
Pages (from-to)1460-1470
Number of pages11
JournalFree Radical Biology and Medicine
Volume36
Issue number11
DOIs
StatePublished - Jun 1 2004

Keywords

  • 2,4-dinitrophenylhydrazine
  • CCCP
  • DNP
  • EB
  • Free radicals
  • Hyperoxia
  • Mitochondria
  • MtDNA
  • Oxygen tolerance
  • Reactive oxygen species
  • Respiration
  • carbonyl cyanide m-chlorophenylhydrazone
  • ethidium bromide
  • respiration-deficient cells
  • ρ°cells

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