Mirror-symmetric microtubule assembly and cell interactions drive lumen formation in the zebrafish neural rod

Clare E. Buckley, Xiaoyun Ren, Laura C. Ward, Gemma C. Girdler, Claudio Araya, Mary J. Green, Brian S. Clark, Brian A. Link, Jonathan D.W. Clarke

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

By analysing the cellular and subcellular events that occur in the centre of the developing zebrafish neural rod, we have uncovered a novel mechanism of cell polarisation during lumen formation. Cells from each side of the neural rod interdigitate across the tissue midline. This is necessary for localisation of apical junctional proteins to the region where cells intersect the tissue midline. Cells assemble a mirror-symmetric microtubule cytoskeleton around the tissue midline, which is necessary for the trafficking of proteins required for normal lumen formation, such as partitioning defective 3 and Rab11a to this point. This occurs in advance and is independent of the midline cell division that has been shown to have a powerful role in lumen organisation. To our knowledge, this is the first example of the initiation of apical polarisation part way along the length of a cell, rather than at a cell extremity. Although the midline division is not necessary for apical polarisation, it confers a morphogenetic advantage by efficiently eliminating cellular processes that would otherwise bridge the developing lumen.

Original languageEnglish
Pages (from-to)30-44
Number of pages15
JournalEMBO Journal
Volume32
Issue number1
DOIs
StatePublished - Jan 9 2013
Externally publishedYes

Keywords

  • Pard3
  • Rab11a
  • apical polarization
  • lumen formation
  • zebrafish

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    Buckley, C. E., Ren, X., Ward, L. C., Girdler, G. C., Araya, C., Green, M. J., Clark, B. S., Link, B. A., & Clarke, J. D. W. (2013). Mirror-symmetric microtubule assembly and cell interactions drive lumen formation in the zebrafish neural rod. EMBO Journal, 32(1), 30-44. https://doi.org/10.1038/emboj.2012.305