miR-34a and miR-125b are upregulated in peripheral blood mononuclear cells from patients with type 2 diabetes mellitus

Yanxin Shen, Huiling Xu, Xiaoyuan Pan, Weijiang Wu, Hui Wang, Linlin Yan, Miaomiao Zhang, Xia Liu, Sheng Xia, Qixiang Shao

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Type 2 diabetes mellitus (T2DM) is a leading cause of blindness, non-traumatic amputation and end-stage renal disease, as well as a major cardiovascular risk factor. To determine whether miR-125b and miR-34a serve an important role in the development of T2DM, the current study investigated the expression profile of two microRNAs (miR-34a and miR-125b) and their relative genes in peripheral blood mononuclear cells from 73 patients with T2DM and 52 healthy donors by reverse transcription-quantitative polymerase chain reaction In addition, the association between miR-34a, miR-125b and their relevant genes expression profile were analyzed with respect to the pathogenesis of T2DM. The present study demonstrated that the expression levels of miR-125b and miR-34a were elevated in peripheral blood mononuclear cell samples from patients with T2DM. Furthermore, miR-34a and miR-125b were positively correlated with low-density lipopro-tein/high-density lipoprotein (HDL) and Foxp3 and negatively related to triglyceride/HDL. However, no correlation among miR-34a, miR-125b and the value of homeostasis model assessment of insulin resistance, homeostasis model assessment of β-cell function and the genes of B lymphocyte-induced maturation protein-1, interferon regulatory factor-4, P53 and retinoid-related orphan receptor γt were observed. These results indicate that the alteration of miR-34a and miR-125b exists in patients with T2DM, which may be involved in the pathogenesis of T2DM, and could be a potential novel biomarker of T2DM.

Original languageEnglish
Pages (from-to)5589-5596
Number of pages8
JournalExperimental and Therapeutic Medicine
Volume14
Issue number6
DOIs
StatePublished - Dec 2017

Keywords

  • B lymphocyte-induced maturation protein-1
  • Forkhead box protein 3
  • Interferon regulatory protein-4 transcription factors
  • MiR-125b
  • MiR-34a
  • P53
  • Peripheral blood mononuclear cells
  • Retinoid-related orphan receptor γt
  • Type 2 diabetes mellitus

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