Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

MISEV Consortium, Joshua A. Welsh, Deborah C.I. Goberdhan, Lorraine O’Driscoll, Edit I. Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom A.P. Driedonks, Uta Erdbrügger, Juan M. Falcon-Perez, Qing Ling Fu, Andrew F. Hill, Metka Lenassi, Sai Kiang Lim, M. ỹ.G. Mahoney, Sujata Mohanty, Andreas Möller, Rienk NieuwlandTakahiro Ochiya, Susmita Sahoo, Ana C. Torrecilhas, Lei Zheng, Andries Zijlstra, Sarah Abuelreich, Reem Bagabas, Paolo Bergese, Esther M. Bridges, Marco Brucale, Dylan Burger, Randy P. Carney, Emanuele Cocucci, Rossella Crescitelli, Edveena Hanser, Adrian L. Harris, Norman J. Haughey, An Hendrix, Alexander R. Ivanov, Tijana Jovanovic-Talisman, Nicole A. Kruh-Garcia, Vroniqa Ku’ulei Lyn Faustino, Diego Kyburz, Cecilia Lässer, Kathleen M. Lennon, Jan Lötvall, Adam L. Maddox, Elena S. Martens-Uzunova, Rachel R. Mizenko, Lauren A. Newman, Andrea Ridolfi, Eva Rohde, Tatu Rojalin, Andrew Rowland, Andras Saftics, Ursula S. Sandau, Julie A. Saugstad, Faezeh Shekari, Simon Swift, Dmitry Ter-Ovanesyan, Juan P. Tosar, Zivile Useckaite, Francesco Valle, Zoltan Varga, Edwin van der Pol, Martijn J.C. van Herwijnen, Marca H.M. Wauben, Ann M. Wehman, Sarah Williams, Andrea Zendrini, Alan J. Zimmerman, Clotilde Théry, Kenneth W. Witwer, Samar Ahmad, Dina A.K. Ahmed, Sarah H. Ahmed, Elena Aikawa, Naveed Akbar, Kazunari Akiyoshi, David P. Al-Adra, Maimonah E. Al-Masawa, Manuel Albanese, Ainhoa Alberro, María José Alcaraz, Jen Alexander-Brett, Kimberley L. Alexander, Nilufar Ali, Faisal J. Alibhai, Susann Allelein, Mark C. Allenby, Fausto Almeida, Luis Pereira de Almeida, Sameh W. Almousa, Nihal Altan-Bonnet, Wanessa F. Altei, Gloria Alvarez-Llamas, Cora L. Alvarez, Hyo Jung An, Krishnan Anand, Samir E.L. Andaloussi, Johnathon D. Anderson, Ramaroson Andriantsitohaina, Khairul I. Ansari, Achille Anselmo, Anna Antoniou, Farrukh Aqil, Tanina Arab, Fabienne Archer, Syrine Arif, David A. Armstrong, Onno J. Arntz, Pierre Arsène, Luis Arteaga-Blanco, Nandini Asokan, Trude Aspelin, Georgia K. Atkin-Smith, Dimitri Aubert, Kanchana K. Ayyar, Maryam Azlan, Ioannis Azoidis, Anaïs Bécot, Jean Marie Bach, Daniel Bachurski, Seoyoon Bae, Roger Olofsson Bagge, Monika Baj-Krzyworzeka, Leonora Balaj, Carolina Balbi, Bas W.M. van Balkom, Abhijna R. Ballal, Afsareen Bano, Sébastien Banzet, Yonis Bare, Lucio Barile, Bahnisikha Barman, Isabel Barranco, Valeria Barreca, Geneviève Bart, Natasha S. Barteneva, Manuela Basso, Mona Batish, Natalie R. Bauer, Amy A. Baxter, Wilfried W. Bazié, Erica Bazzan, Joel E.J. Beaumont, Mary Bebawy, Maarten P. Bebelman, Apolonija Bedina-Zavec, Danielle J. Beetler, Tamás Beke-Somfai, Clémence Belleannée, Birke J. Benedikter, Berglind E. Benediktsdóttir, Anna C. Berardi, Mathilde Bergamelli, Irene Bertolini, Asima Bhattacharyya, Suvendra N. Bhattacharyya, Steven J. Biller, Clotilde Billottet, John J. Bissler, Olivier Blanc-Brude, Charles J. Blijdorp, Sylwia Bobis-Wozowicz, Victor Bodart-Santos, Bernadett R. Bodnár, Eric Boilard, Wilfrid Boireau, Vladimir Bokun, Stephanie M. Bollard, Sveva Bollini, Antonella Bongiovanni, Laura Bongiovanni, Amandine Bonifay, Marni D. Boppart, Francesc E. Borràs, Steffi Bosch, Daniela Boselli, Massimo Bottini, Jeff Bouffard, Chantal M. Boulanger, Paul C. Boutros, Oscar Boyadjian, Anders T. Boysen, Batuhan T. Bozkurt, Kyle P. Bramich, Fabian Braun, Rocío Del Carmen Bravo-Miana, Xandra O. Breakefield, Santra Brenna, Kieran Brennan, Meadhbh Brennan, Koen Breyne, David R. Brigstock, Alain R. Brisson, Chaya Brodie, Katelyn A. Bruno, Cecilia Bucci, Shilpa Buch, Amyh Buck

Research output: Contribution to journalArticlepeer-review

458 Scopus citations

Abstract

Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year-on-year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non-vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

Original languageEnglish
Article numbere12404
JournalJournal of Extracellular Vesicles
Volume13
Issue number2
DOIs
StatePublished - Feb 2024

Keywords

  • MISEV
  • ectosomes
  • exosomes
  • extracellular particles
  • extracellular vesicles
  • guidelines
  • microparticles
  • microvesicles
  • minimal information requirements
  • reproducibility
  • rigor
  • standardisation

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