Miniaturizing sample spots for matrix-assisted laser desorption/ionization mass spectrometry

Tingting Tu; Michael L. Gross

doi:10.1016/j.trac.2009.03.013

Miniaturizing sample spots for matrix-assisted laser desorption/ionization mass spectrometry

Tingting Tu, Michael L. Gross

Research output: Contribution to journal › Review article › peer-review

4 Scopus citations

Abstract

The trend of miniaturization in bioanalytical chemistry is shifting from technical development to practical application. In matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), progress in miniaturizing sample spots has been driven by the needs to increase sensitivity and speed, to interface with other analytical microtechnologies, and to develop miniaturized instrumentation. We review recent developments in miniaturizing sample spots for MALDI-MS. We cover both target modification and microdispensing technologies, and we emphasize the benefits with respect to sensitivity, throughput and automation. We hope that this review will encourage further method development and application of miniaturized sample spots for MALDI-MS, so as to expand applications in analytical chemistry, protein science and molecular biology.

Original language	English
Pages (from-to)	833-841
Number of pages	9
Journal	TrAC - Trends in Analytical Chemistry
Volume	28
Issue number	7
DOIs	https://doi.org/10.1016/j.trac.2009.03.013
State	Published - Aug 2009

Keywords

Acoustic ejection
Hydrophobic surface
Induction-based fluidics
Mass spectrometry
Matrix-assisted laser desorption/ionization (MALDI)
Microdispensing
Miniaturization
Nanovial
Piezoelectric dispensing
Sample preparation

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10.1016/j.trac.2009.03.013

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title = "Miniaturizing sample spots for matrix-assisted laser desorption/ionization mass spectrometry",

abstract = "The trend of miniaturization in bioanalytical chemistry is shifting from technical development to practical application. In matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), progress in miniaturizing sample spots has been driven by the needs to increase sensitivity and speed, to interface with other analytical microtechnologies, and to develop miniaturized instrumentation. We review recent developments in miniaturizing sample spots for MALDI-MS. We cover both target modification and microdispensing technologies, and we emphasize the benefits with respect to sensitivity, throughput and automation. We hope that this review will encourage further method development and application of miniaturized sample spots for MALDI-MS, so as to expand applications in analytical chemistry, protein science and molecular biology.",

keywords = "Acoustic ejection, Hydrophobic surface, Induction-based fluidics, Mass spectrometry, Matrix-assisted laser desorption/ionization (MALDI), Microdispensing, Miniaturization, Nanovial, Piezoelectric dispensing, Sample preparation",

author = "Tingting Tu and Gross, {Michael L.}",

note = "Funding Information: Financial support from the National Centers for Research Resources of the NIH under Grant No. P41RR000954 is gratefully acknowledged.",

year = "2009",

month = aug,

doi = "10.1016/j.trac.2009.03.013",

language = "English",

volume = "28",

pages = "833--841",

journal = "TrAC - Trends in Analytical Chemistry",

issn = "0165-9936",

number = "7",

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TY - JOUR

T1 - Miniaturizing sample spots for matrix-assisted laser desorption/ionization mass spectrometry

AU - Tu, Tingting

AU - Gross, Michael L.

N1 - Funding Information: Financial support from the National Centers for Research Resources of the NIH under Grant No. P41RR000954 is gratefully acknowledged.

PY - 2009/8

Y1 - 2009/8

N2 - The trend of miniaturization in bioanalytical chemistry is shifting from technical development to practical application. In matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), progress in miniaturizing sample spots has been driven by the needs to increase sensitivity and speed, to interface with other analytical microtechnologies, and to develop miniaturized instrumentation. We review recent developments in miniaturizing sample spots for MALDI-MS. We cover both target modification and microdispensing technologies, and we emphasize the benefits with respect to sensitivity, throughput and automation. We hope that this review will encourage further method development and application of miniaturized sample spots for MALDI-MS, so as to expand applications in analytical chemistry, protein science and molecular biology.

AB - The trend of miniaturization in bioanalytical chemistry is shifting from technical development to practical application. In matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), progress in miniaturizing sample spots has been driven by the needs to increase sensitivity and speed, to interface with other analytical microtechnologies, and to develop miniaturized instrumentation. We review recent developments in miniaturizing sample spots for MALDI-MS. We cover both target modification and microdispensing technologies, and we emphasize the benefits with respect to sensitivity, throughput and automation. We hope that this review will encourage further method development and application of miniaturized sample spots for MALDI-MS, so as to expand applications in analytical chemistry, protein science and molecular biology.

KW - Acoustic ejection

KW - Hydrophobic surface

KW - Induction-based fluidics

KW - Mass spectrometry

KW - Matrix-assisted laser desorption/ionization (MALDI)

KW - Microdispensing

KW - Miniaturization

KW - Nanovial

KW - Piezoelectric dispensing

KW - Sample preparation

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U2 - 10.1016/j.trac.2009.03.013

DO - 10.1016/j.trac.2009.03.013

M3 - Review article

AN - SCOPUS:67651115641

SN - 0165-9936

VL - 28

SP - 833

EP - 841

JO - TrAC - Trends in Analytical Chemistry

JF - TrAC - Trends in Analytical Chemistry

IS - 7

ER -

Miniaturizing sample spots for matrix-assisted laser desorption/ionization mass spectrometry

Abstract

Keywords

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