Miniaturization and defocus correction for objective-coupled planar illumination microscopy

Diwakar Turaga, Timothy E. Holy

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Recently, a light sheet-based technique called objective-coupled planar illumination (OCPI) microscopy [Holekamp et al., Neuron 57, 661 (2008)] was shown to permit low-phototoxicity, high-speed, threedimensional fluorescence imaging of extended tissue samples. Here, we introduce two major improvements in OCPI microscopy. First, we miniaturize the objective coupler by using a uniaxial gradient-index lens to produce the light sheet. Second, we demonstrate theoretically and experimentally that refractive index mismatch at the fluid/tissue interface causes a significant defocus aberration. By introducing the ability to tune the angle of the light sheet, we show that defocus correction in a miniaturized OCPI microscope leads to a significant improvement in image sharpness deeper into tissue.

Original languageEnglish
Pages (from-to)2302-2304
Number of pages3
JournalOptics Letters
Issue number20
StatePublished - Oct 15 2008


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