TY - JOUR
T1 - Migration of Zebrafish Primordial Germ Cells
T2 - A Role for Myosin Contraction and Cytoplasmic Flow
AU - Blaser, Heiko
AU - Reichman-Fried, Michal
AU - Castanon, Irinka
AU - Dumstrei, Karin
AU - Marlow, Florence L L.
AU - Kawakami, Koichi
AU - Solnica-Krezel, Lilianna
AU - Heisenberg, Carl Philipp
AU - Raz, Erez
N1 - Funding Information:
We thank Rex Chisholm, Steve Farber, Adi Kimchi, Masamitsu Iino, Klemens Rottner, Heikki Rauvala, and Marcus Fechheimer for kindly providing us with DNA constructs and Helen Le-Cordier and Julia Dörries for technical assistance. We are grateful to Elena Kardash for helpful discussions and to Sonia Minina and Ewa Paluch for critical reading of the manuscript. This work was supported by funds from the Max Planck Society and grants from the Deutsche Forschungsgemeinschaft to E.R. and to C.-P.H. H.B. was supported by Roche Research Foundation, I.C. by the Humboldt Foundation, K.D. by a European Molecular Biology Organization long-term fellowship, and L.S.K. by National Institutes of Health grants GM55101 and GM 77770.
PY - 2006/11
Y1 - 2006/11
N2 - The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.
AB - The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.
KW - CELLBIO
KW - DEVBIO
KW - STEMCELL
UR - http://www.scopus.com/inward/record.url?scp=33750491282&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2006.09.023
DO - 10.1016/j.devcel.2006.09.023
M3 - Article
C2 - 17084355
AN - SCOPUS:33750491282
SN - 1534-5807
VL - 11
SP - 613
EP - 627
JO - Developmental cell
JF - Developmental cell
IS - 5
ER -