Abstract
Histones are chromatin proteins that are highly modified with many different types of post-translational modifications. These modifications act in concert to regulate a number of chromatin-related processes. However, identification and quantification of co-occurring histone post-translational modifications is challenging because there are many potential combinations of modifications and because the commonly used strategy of fragmenting proteins using trypsin or an alternative protease prior to LC-MS/MS analysis results in the loss of connectivity between modifications on different peptides. In this unit, mass spectrometric methods to analyze combinatorial histone modifications on histone tails (middle-down mass spectrometry) and on intact histones (top-down mass spectrometry) are described.
Original language | English |
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Pages (from-to) | 23.7.1-23.7.28 |
Journal | Current Protocols in Protein Science |
Volume | 2014 |
DOIs | |
State | Published - 2014 |
Keywords
- Histone modifications
- Middle-down mass spectrometry
- Post-translational modifications
- Top-down mass spectrometry