Microtubules provide directional information for core PCP function

Maja Matis, David A. Russler-Germain, Qie Hu, Claire J. Tomlin, Jeffrey D. Axelrod

Research output: Contribution to journalArticlepeer-review

93 Scopus citations


Planar cell polarity (PCP) signaling controls the polarization of cells within the plane of an epithelium. Two molecular modules composed of Fat(Ft)/Dachsous(Ds)/Four-jointed(Fj) and a ‘PCP-core’ including Frizzled(Fz) and Dishevelled(Dsh) contribute to polarization of individual cells. How polarity is globally coordinated with tissue axes is unresolved. Consistent with previous results, we find that the Ft/Ds/Fj-module has an effect on a MT-cytoskeleton. Here, we provide evidence for the model that the Ft/Ds/Fj-module provides directional information to the coremodule through this MT organizing function. We show Ft/Ds/Fj-dependent initial polarization of the apical MT-cytoskeleton prior to global alignment of the core-module, reveal that the anchoring of apical non-centrosomal MTs at apical junctions is polarized, observe that directional trafficking of vesicles containing Dsh depends on Ft, and demonstrate the feasibility of this model by mathematical simulation. Together, these results support the hypothesis that Ft/Ds/Fj provides a signal to orient core PCP function via MT polarization.

Original languageEnglish
Article numbere02893
Pages (from-to)1-17
Number of pages17
Issue numberAugust2014
StatePublished - Aug 14 2014


Dive into the research topics of 'Microtubules provide directional information for core PCP function'. Together they form a unique fingerprint.

Cite this