Microsporidian Spore Discharge and the Transfer of Polaroplast Organelle Membrane into Plasma Membrane

EARL WEIDNER, WILLIAM BYRD, ANN SCARBOROUGH, JOHN PLESHINGER, DAVID SIBLEY

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Electron microscopic examinations of Glugea hertwigi and Spraguea lophii spores indicated the presence of a single plasma membrane; however, this membrane remained in the spore during the discharge of the sporoplasm from the spore. Although discharged spores retained the old plasma membrane, the extruded sporoplasms acquired a new plasma membrane. In order to determine where the new plasma membrane came from, we used two fluorescent probes with membrane affinities. The markers were tested on unfired and discharged spores. The probe, N‐phenyl‐1‐naphthylamine (NPN), labeled the polaroplast membrane in addition to the apolar groups in the posterior vacuoles of unfired spores. After spore discharge, NPN label disappeared from the spore ghosts except for a slight fluorescence on residual plasma membranes. Much of the NPN‐labeled membrane reappeared after spore discharge on the outer envelope of discharged sporoplasms. The probe chlorotetracycline (CTC) labeled calcium‐associated membranes of spore polaroplasts. During spore discharge, the CTC fluorescence shifted from the polaroplast organelle of unfired spores to the outer envelope of discharged sporoplasms. These results indicate that the polaroplast organelle may provide the new plasma membrane for discharged microsporidian sporoplasms.

Original languageEnglish
Pages (from-to)195-198
Number of pages4
JournalThe Journal of Protozoology
Volume31
Issue number2
DOIs
StatePublished - May 1984
Externally publishedYes

Fingerprint Dive into the research topics of 'Microsporidian Spore Discharge and the Transfer of Polaroplast Organelle Membrane into Plasma Membrane'. Together they form a unique fingerprint.

  • Cite this