Microscopy assays for evaluation of mast cell Migration and Chemotaxis

Monika Bambousková, Zuzana Hájková, Pavel Dráber, Petr Dráber

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A better understanding of the molecular mechanisms leading to mast cell migration and chemotaxis is the long-term goal in mast cell research and is essential for comprehension of mast cell function in health and disease. Various techniques have been developed in recent decades for in vitro and in vivo assessment of mast cell motility and chemotaxis. In this chapter three microscopy assays facilitating real-time quantification of mast cell chemotaxis and migration are described, focusing on individual cell tracking and data analysis.

Original languageEnglish
Pages (from-to)161-176
Number of pages16
JournalMethods in Molecular Biology
Volume1192
DOIs
StatePublished - 2014

Keywords

  • Cell migration
  • Chemoattractant
  • Chemokine
  • Chemotaxis
  • Mast cells

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