TY - JOUR
T1 - Microscopic Investigation of Protein Function in C. elegans Using Fluorescent Imaging
AU - Luke, Cliff J.
AU - O'Reilly, Linda P.
PY - 2015/10/1
Y1 - 2015/10/1
N2 - Caenorhabditis elegans is a powerful model organism for studying human biology and disease due to its surprisingly high genetic homology to Homo sapiens. Its genetic amenability, small size, short generation time, and transparent body make it an ideal organism for multiple scientific disciplines. Fluorescent microscopy is essential for studying protein biological function. However, C. elegans, mainly due to its high motility, has been more difficult to adapt to fluorescence imaging, especially live-imaging. We present here several protocols for the study of protein location, function and dynamics in context of a whole animal. These protocols, especially when combined with existing genetic procedures, can yield a great deal of insight in the physiological roles of proteins in C. elegans, which can be directly translated into mammalian systems.
AB - Caenorhabditis elegans is a powerful model organism for studying human biology and disease due to its surprisingly high genetic homology to Homo sapiens. Its genetic amenability, small size, short generation time, and transparent body make it an ideal organism for multiple scientific disciplines. Fluorescent microscopy is essential for studying protein biological function. However, C. elegans, mainly due to its high motility, has been more difficult to adapt to fluorescence imaging, especially live-imaging. We present here several protocols for the study of protein location, function and dynamics in context of a whole animal. These protocols, especially when combined with existing genetic procedures, can yield a great deal of insight in the physiological roles of proteins in C. elegans, which can be directly translated into mammalian systems.
KW - Caenorhabditis elegans
KW - antibody
KW - fluorescence microscopy
KW - fluorescent protein
KW - immobilization
KW - time-lapse imaging
UR - http://www.scopus.com/inward/record.url?scp=84987600876&partnerID=8YFLogxK
U2 - 10.1002/0471142956.cy1241s74
DO - 10.1002/0471142956.cy1241s74
M3 - Article
C2 - 26423692
AN - SCOPUS:84987600876
VL - 74
SP - 12.41.1-12.41.17
JO - Current Protocols in Cytometry
JF - Current Protocols in Cytometry
SN - 1934-9297
IS - 1
ER -