Microscale preparation of natural and labeled oxysterols

Louis H. Krut, Gustav Schonfeld, Richard E. Ostlund

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The preparation of cholesterol and radiocholesterol oxidation products on a microscale is difficult. Cholesterol generally resists oxidation unless it is well dispersed under controlled conditions. A method was developed to reliably produce 7α- and 7β-hydroxycholesterol and 7-ketocholesterol. Small changes in pH, metal ions present, or in the colloidal dispersion, resulted in production of completely different oxysterols. Attempts to oxidize aged radiocholesterol were not successful even after purification by several thin- layer chromatographic steps, and this appeared to be due to a time related change in the radioactive material. Fresh radiocholesterol oxidized readily.

Original languageEnglish
Pages (from-to)529-534
Number of pages6
Issue number5
StatePublished - May 1996


Dive into the research topics of 'Microscale preparation of natural and labeled oxysterols'. Together they form a unique fingerprint.

Cite this