MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression

Jennifer H. Mansfield, Brian D. Harfe, Robert Nissen, John Obenauer, Jalagani Srineel, Aadel Chaudhuri, Raphael Farzan-Kashani, Michael Zuker, Amy E. Pasquinelli, Gary Ruvkun, Phillip A. Sharp, Clifford J. Tabin, Michael T. McManus

Research output: Contribution to journalArticle

356 Scopus citations

Abstract

MicroRNAs (miRNAs) are a class of short (∼22-nt) noncoding RNA molecules that downregulate expression of their mRNA targets. Since their discovery as regulators of developmental timing in Caenorhabditis elegans, hundreds of miRNAs have been identified in both animals and plants. Here, we report a technique for visualizing detailed miRNA expression patterns in mouse embryos. We elucidate the tissue-specific expression of several miRNAs during embryogenesis, including two encoded by genes embedded in homeobox (Hox) clusters, miR-10a and miR-196a. These two miRNAs are expressed in patterns that are markedly reminiscent of those of Hox genes. Furthermore, miR-196a negatively regulates Hoxh8, indicating that its restricted expression pattern probably reflects a role in the patterning function of the Hox complex.

Original languageEnglish
Pages (from-to)1079-1083
Number of pages5
JournalNature Genetics
Volume36
Issue number10
DOIs
StatePublished - Oct 1 2004
Externally publishedYes

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    Mansfield, J. H., Harfe, B. D., Nissen, R., Obenauer, J., Srineel, J., Chaudhuri, A., Farzan-Kashani, R., Zuker, M., Pasquinelli, A. E., Ruvkun, G., Sharp, P. A., Tabin, C. J., & McManus, M. T. (2004). MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression. Nature Genetics, 36(10), 1079-1083. https://doi.org/10.1038/ng1421