MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression

Jennifer H. Mansfield; Brian D. Harfe; Robert Nissen; John Obenauer; Jalagani Srineel; Aadel Chaudhuri; Raphael Farzan-Kashani; Michael Zuker; Amy E. Pasquinelli; Gary Ruvkun; Phillip A. Sharp; Clifford J. Tabin; Michael T. McManus

doi:10.1038/ng1421

MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression

Jennifer H. Mansfield, Brian D. Harfe, Robert Nissen, John Obenauer, Jalagani Srineel, Aadel Chaudhuri, Raphael Farzan-Kashani, Michael Zuker, Amy E. Pasquinelli, Gary Ruvkun, Phillip A. Sharp, Clifford J. Tabin, Michael T. McManus

Research output: Contribution to journal › Article › peer-review

372 Scopus citations

Abstract

MicroRNAs (miRNAs) are a class of short (∼22-nt) noncoding RNA molecules that downregulate expression of their mRNA targets. Since their discovery as regulators of developmental timing in Caenorhabditis elegans, hundreds of miRNAs have been identified in both animals and plants. Here, we report a technique for visualizing detailed miRNA expression patterns in mouse embryos. We elucidate the tissue-specific expression of several miRNAs during embryogenesis, including two encoded by genes embedded in homeobox (Hox) clusters, miR-10a and miR-196a. These two miRNAs are expressed in patterns that are markedly reminiscent of those of Hox genes. Furthermore, miR-196a negatively regulates Hoxh8, indicating that its restricted expression pattern probably reflects a role in the patterning function of the Hox complex.

Original language	English
Pages (from-to)	1079-1083
Number of pages	5
Journal	Nature Genetics
Volume	36
Issue number	10
DOIs	https://doi.org/10.1038/ng1421
State	Published - Oct 2004

Access to Document

10.1038/ng1421

Cite this

Mansfield, J. H., Harfe, B. D., Nissen, R., Obenauer, J., Srineel, J., Chaudhuri, A., Farzan-Kashani, R., Zuker, M., Pasquinelli, A. E., Ruvkun, G., Sharp, P. A., Tabin, C. J., & McManus, M. T. (2004). MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression. Nature Genetics, 36(10), 1079-1083. https://doi.org/10.1038/ng1421

@article{66caeaa83e3647d9b60b73d5bc9e5f26,

title = "MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression",

abstract = "MicroRNAs (miRNAs) are a class of short (∼22-nt) noncoding RNA molecules that downregulate expression of their mRNA targets. Since their discovery as regulators of developmental timing in Caenorhabditis elegans, hundreds of miRNAs have been identified in both animals and plants. Here, we report a technique for visualizing detailed miRNA expression patterns in mouse embryos. We elucidate the tissue-specific expression of several miRNAs during embryogenesis, including two encoded by genes embedded in homeobox (Hox) clusters, miR-10a and miR-196a. These two miRNAs are expressed in patterns that are markedly reminiscent of those of Hox genes. Furthermore, miR-196a negatively regulates Hoxh8, indicating that its restricted expression pattern probably reflects a role in the patterning function of the Hox complex.",

author = "Mansfield, {Jennifer H.} and Harfe, {Brian D.} and Robert Nissen and John Obenauer and Jalagani Srineel and Aadel Chaudhuri and Raphael Farzan-Kashani and Michael Zuker and Pasquinelli, {Amy E.} and Gary Ruvkun and Sharp, {Phillip A.} and Tabin, {Clifford J.} and McManus, {Michael T.}",

note = "Funding Information: We thank S. Dymecki for plasmids; A. Abney and L. Du for pronuclear injections of the transgenes; members of the laboratories of C.J.T., C. Cepko, S. Dymecki and P.A.S. for discussions and advice; and A. Brent for critical reading of the manuscript. This work was supported by a grant from the March of Dimes to C.J.T., and by a US National Institutes of Health MERIT award and grants from the National Cancer Institute to P.A.S. J.H.M. and M.T.M. are supported by postdoctoral fellowships from the National Institutes of Health and the Cancer Research Institute, respectively.",

year = "2004",

month = oct,

doi = "10.1038/ng1421",

language = "English",

volume = "36",

pages = "1079--1083",

journal = "Nature Genetics",

issn = "1061-4036",

number = "10",

}

Mansfield, JH, Harfe, BD, Nissen, R, Obenauer, J, Srineel, J, Chaudhuri, A, Farzan-Kashani, R, Zuker, M, Pasquinelli, AE, Ruvkun, G, Sharp, PA, Tabin, CJ & McManus, MT 2004, 'MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression', Nature Genetics, vol. 36, no. 10, pp. 1079-1083. https://doi.org/10.1038/ng1421

TY - JOUR

T1 - MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression

AU - Mansfield, Jennifer H.

AU - Harfe, Brian D.

AU - Nissen, Robert

AU - Obenauer, John

AU - Srineel, Jalagani

AU - Chaudhuri, Aadel

AU - Farzan-Kashani, Raphael

AU - Zuker, Michael

AU - Pasquinelli, Amy E.

AU - Ruvkun, Gary

AU - Sharp, Phillip A.

AU - Tabin, Clifford J.

AU - McManus, Michael T.

N1 - Funding Information: We thank S. Dymecki for plasmids; A. Abney and L. Du for pronuclear injections of the transgenes; members of the laboratories of C.J.T., C. Cepko, S. Dymecki and P.A.S. for discussions and advice; and A. Brent for critical reading of the manuscript. This work was supported by a grant from the March of Dimes to C.J.T., and by a US National Institutes of Health MERIT award and grants from the National Cancer Institute to P.A.S. J.H.M. and M.T.M. are supported by postdoctoral fellowships from the National Institutes of Health and the Cancer Research Institute, respectively.

PY - 2004/10

Y1 - 2004/10

N2 - MicroRNAs (miRNAs) are a class of short (∼22-nt) noncoding RNA molecules that downregulate expression of their mRNA targets. Since their discovery as regulators of developmental timing in Caenorhabditis elegans, hundreds of miRNAs have been identified in both animals and plants. Here, we report a technique for visualizing detailed miRNA expression patterns in mouse embryos. We elucidate the tissue-specific expression of several miRNAs during embryogenesis, including two encoded by genes embedded in homeobox (Hox) clusters, miR-10a and miR-196a. These two miRNAs are expressed in patterns that are markedly reminiscent of those of Hox genes. Furthermore, miR-196a negatively regulates Hoxh8, indicating that its restricted expression pattern probably reflects a role in the patterning function of the Hox complex.

AB - MicroRNAs (miRNAs) are a class of short (∼22-nt) noncoding RNA molecules that downregulate expression of their mRNA targets. Since their discovery as regulators of developmental timing in Caenorhabditis elegans, hundreds of miRNAs have been identified in both animals and plants. Here, we report a technique for visualizing detailed miRNA expression patterns in mouse embryos. We elucidate the tissue-specific expression of several miRNAs during embryogenesis, including two encoded by genes embedded in homeobox (Hox) clusters, miR-10a and miR-196a. These two miRNAs are expressed in patterns that are markedly reminiscent of those of Hox genes. Furthermore, miR-196a negatively regulates Hoxh8, indicating that its restricted expression pattern probably reflects a role in the patterning function of the Hox complex.

UR - http://www.scopus.com/inward/record.url?scp=6944246368&partnerID=8YFLogxK

U2 - 10.1038/ng1421

DO - 10.1038/ng1421

M3 - Article

C2 - 15361871

AN - SCOPUS:6944246368

SN - 1061-4036

VL - 36

SP - 1079

EP - 1083

JO - Nature Genetics

JF - Nature Genetics

IS - 10

ER -

MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression

Abstract

Access to Document

Other files and links

Fingerprint

Cite this