MicroRNA and mRNA expression profile screening in multiple sclerosis patients to unravel novel pathogenic steps and identify potential biomarkers

Filippo Martinelli-Boneschi, Chiara Fenoglio, Paola Brambilla, Melissa Sorosina, Giacomo Giacalone, Federica Esposito, Maria Serpente, Claudia Cantoni, Elisa Ridolfi, Mariaemma Rodegher, Lucia Moiola, Bruno Colombo, Milena De Riz, Vittorio Martinelli, Elio Scarpini, Giancarlo Comi, Daniela Galimberti

Research output: Contribution to journalComment/debate

61 Scopus citations

Abstract

Identification of novel targets and biomarkers, such as microRNAs, is extremely helpful to understand the pathogenetic mechanisms in a disease like multiple sclerosis (MS). We tested the expression profile of 1145 microRNAs in peripheral blood mononuclear cells (PBMCs) of 19 MS patients and 14 controls, and we further explored their function by performing a whole-genome mRNA profiling in same subjects and using bioinformatic prediction tool. A total of 104 miRNAs have been identified as deregulated in MS patients; 2/10 which ranked highest (let-7g and miR-150) have been validated in a replication sample, leading to the identification of putative target genes.

Original languageEnglish
Pages (from-to)4-8
Number of pages5
JournalNeuroscience Letters
Volume508
Issue number1
DOIs
StatePublished - Feb 2 2012
Externally publishedYes

Keywords

  • Gene expression
  • MicroRNA
  • Multiple sclerosis

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