TY - JOUR
T1 - Microbial Synthesis of High-Molecular-Weight, Highly Repetitive Protein Polymers
AU - Jeon, Juya
AU - Subramani, Shri Venkatesh
AU - Lee, Kok Zhi
AU - Jiang, Bojing
AU - Zhang, Fuzhong
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/4
Y1 - 2023/4
N2 - High molecular weight (MW), highly repetitive protein polymers are attractive candidates to replace petroleum-derived materials as these protein-based materials (PBMs) are renewable, biodegradable, and have outstanding mechanical properties. However, their high MW and highly repetitive sequence features make them difficult to synthesize in fast-growing microbial cells in sufficient amounts for real applications. To overcome this challenge, various methods were developed to synthesize repetitive PBMs. Here, we review recent strategies in the construction of repetitive genes, expression of repetitive proteins from circular mRNAs, and synthesis of repetitive proteins by ligation and protein polymerization. We discuss the advantages and limitations of each method and highlight future directions that will lead to scalable production of highly repetitive PBMs for a wide range of applications.
AB - High molecular weight (MW), highly repetitive protein polymers are attractive candidates to replace petroleum-derived materials as these protein-based materials (PBMs) are renewable, biodegradable, and have outstanding mechanical properties. However, their high MW and highly repetitive sequence features make them difficult to synthesize in fast-growing microbial cells in sufficient amounts for real applications. To overcome this challenge, various methods were developed to synthesize repetitive PBMs. Here, we review recent strategies in the construction of repetitive genes, expression of repetitive proteins from circular mRNAs, and synthesis of repetitive proteins by ligation and protein polymerization. We discuss the advantages and limitations of each method and highlight future directions that will lead to scalable production of highly repetitive PBMs for a wide range of applications.
KW - high molecular weight
KW - microbial synthesis
KW - protein polymers
KW - repetitive proteins
KW - silk
KW - synthetic biology
UR - http://www.scopus.com/inward/record.url?scp=85152531032&partnerID=8YFLogxK
U2 - 10.3390/ijms24076416
DO - 10.3390/ijms24076416
M3 - Review article
C2 - 37047388
AN - SCOPUS:85152531032
SN - 1661-6596
VL - 24
JO - International journal of molecular sciences
JF - International journal of molecular sciences
IS - 7
M1 - 6416
ER -