Abstract

Manipulation of cells using standing surface acoustic waves (SSAW) is useful in many biomedical applications; however, trapping of highly-motile micro-swimmers like Chlamydomonas reinhardtii (CR) is challenging using traditional SSAW devices incorporating elastomeric microchannels. In these devices, generation of the required high trapping force heats samples beyond the thermotolerance threshold of the cells leading to loss of function or death. Herein, we demonstrate trap-and-release of CR using SSAW devices with wet-etched glass channels driven at ~10 and ~25 MHz. Infrared thermography confirms a reduction in operating temperature for glass (versus polydimethylsiloxane, PDMS) channels to achieve sufficient trapping force with improved biocompatibility.

Original languageEnglish
Title of host publication23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PublisherChemical and Biological Microsystems Society
Pages1622-1623
Number of pages2
ISBN (Electronic)9781733419000
StatePublished - 2019
Event23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland
Duration: Oct 27 2019Oct 31 2019

Publication series

Name23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Country/TerritorySwitzerland
CityBasel
Period10/27/1910/31/19

Keywords

  • Acoustofluidics
  • Cell trapping
  • Micro-swimmers
  • Standing surface acoustic waves

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