Micro-RNAs involved in cellular proliferation have altered expression profiles in granulosa of young women with diminished ovarian reserve

Irene Woo, Lane K. Christenson, Sumedha Gunewardena, Sue Ann Ingles, Semara Thomas, Ali Ahmady, Karine Chung, Kristin Bendikson, Richard Paulson, Lynda K. McGinnis

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Purpose: The study aims to determine differences in micro-RNA (miRNA) expression in granulosa (GC) and cumulus cells (CC) between young women with diminished ovarian reserve (DOR) or normal ovarian reserve (NOR). Secondary objective was to identify downstream signaling pathways that could ultimately indicate causes of lower developmental competence of oocytes from young women with DOR. Methods: The method of the study is prospective cohort study. Results: Of the miRNA, 125 are differentially expressed in GC between DOR and NOR. Only nine miRNA were different in CC; therefore, we focused analysis on GC. In DOR GC, miR-100-5p, miR-16-5p, miR-30a-3p, and miR-193a-3p were significantly downregulated, while miR-155-5p, miR-192-5p, miR-128-3p, miR-486-5p, miR130a-3p, miR-92a-3p, miR-17-3p, miR-221-3p, and miR-175p were increased. This pattern predicted higher cell proliferation in the DOR GC. The primary pathways include MAPK, Wnt, and TGFbeta. Conclusions: The miRNA pattern identified critical functions in cell proliferation and survival associated with DOR. GC in women with DOR seems to respond differently to the LH surge.

Original languageEnglish
Pages (from-to)1777-1786
Number of pages10
JournalJournal of Assisted Reproduction and Genetics
Volume35
Issue number10
DOIs
StatePublished - Oct 1 2018

Keywords

  • Cumulus cells
  • Diminished ovarian reserve
  • Granulosa cells
  • Micro-RNA

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