Micelle-Enhanced Chemiluminescence and Application to the Determination of Biological Reductants Using Lucigenin

Willie L. Hinze; Terrence E. Riehl; H. N. Singh; Yoshimi Baba

doi:10.1021/ac00276a046

Micelle-Enhanced Chemiluminescence and Application to the Determination of Biological Reductants Using Lucigenin

Willie L. Hinze, Terrence E. Riehl, H. N. Singh, Yoshimi Baba

Division of Gastroenterology

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

The feasibility of using aqueous surfactant micelles to Improve analytical chemiluminescence (CL) measurements was examined. Specifically, the luclgenin (Luc)-N-methylacrldone CL system was characterized in different micellar and homogeneous media and the effects of various micellar systems upon the CL determination of biological reductants using Luc were assessed. It was found that solubility problems are obviated If the Luc CL assay is conducted In aqueous micelles. Additionally, the presence of cationic or zwltterionlc micelles Improves the sensitivity of the Luc CL assays for ascorbic acid, uric acid, glucose, and fructose by factors of 1.8-4.0. The average precision of the measurements Is ~9% In the micellar media while the accuracy Is comparable to that obtained In water alone. In a few Instances, potential problems due to mutual interference from other reductants can be eliminated via use of micelles. A brief prospective on the general applicability of micellar-enhanced CL In chemical analysis Is given.

Original language	English
Pages (from-to)	2180-2191
Number of pages	12
Journal	Analytical Chemistry
Volume	56
Issue number	12
DOIs	https://doi.org/10.1021/ac00276a046
State	Published - Oct 1984

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title = "Micelle-Enhanced Chemiluminescence and Application to the Determination of Biological Reductants Using Lucigenin",

abstract = "The feasibility of using aqueous surfactant micelles to Improve analytical chemiluminescence (CL) measurements was examined. Specifically, the luclgenin (Luc)-N-methylacrldone CL system was characterized in different micellar and homogeneous media and the effects of various micellar systems upon the CL determination of biological reductants using Luc were assessed. It was found that solubility problems are obviated If the Luc CL assay is conducted In aqueous micelles. Additionally, the presence of cationic or zwltterionlc micelles Improves the sensitivity of the Luc CL assays for ascorbic acid, uric acid, glucose, and fructose by factors of 1.8-4.0. The average precision of the measurements Is ~9% In the micellar media while the accuracy Is comparable to that obtained In water alone. In a few Instances, potential problems due to mutual interference from other reductants can be eliminated via use of micelles. A brief prospective on the general applicability of micellar-enhanced CL In chemical analysis Is given.",

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year = "1984",

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T1 - Micelle-Enhanced Chemiluminescence and Application to the Determination of Biological Reductants Using Lucigenin

AU - Hinze, Willie L.

AU - Riehl, Terrence E.

AU - Singh, H. N.

AU - Baba, Yoshimi

PY - 1984/10

Y1 - 1984/10

N2 - The feasibility of using aqueous surfactant micelles to Improve analytical chemiluminescence (CL) measurements was examined. Specifically, the luclgenin (Luc)-N-methylacrldone CL system was characterized in different micellar and homogeneous media and the effects of various micellar systems upon the CL determination of biological reductants using Luc were assessed. It was found that solubility problems are obviated If the Luc CL assay is conducted In aqueous micelles. Additionally, the presence of cationic or zwltterionlc micelles Improves the sensitivity of the Luc CL assays for ascorbic acid, uric acid, glucose, and fructose by factors of 1.8-4.0. The average precision of the measurements Is ~9% In the micellar media while the accuracy Is comparable to that obtained In water alone. In a few Instances, potential problems due to mutual interference from other reductants can be eliminated via use of micelles. A brief prospective on the general applicability of micellar-enhanced CL In chemical analysis Is given.

AB - The feasibility of using aqueous surfactant micelles to Improve analytical chemiluminescence (CL) measurements was examined. Specifically, the luclgenin (Luc)-N-methylacrldone CL system was characterized in different micellar and homogeneous media and the effects of various micellar systems upon the CL determination of biological reductants using Luc were assessed. It was found that solubility problems are obviated If the Luc CL assay is conducted In aqueous micelles. Additionally, the presence of cationic or zwltterionlc micelles Improves the sensitivity of the Luc CL assays for ascorbic acid, uric acid, glucose, and fructose by factors of 1.8-4.0. The average precision of the measurements Is ~9% In the micellar media while the accuracy Is comparable to that obtained In water alone. In a few Instances, potential problems due to mutual interference from other reductants can be eliminated via use of micelles. A brief prospective on the general applicability of micellar-enhanced CL In chemical analysis Is given.

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Micelle-Enhanced Chemiluminescence and Application to the Determination of Biological Reductants Using Lucigenin

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