Micelle-Enhanced Chemiluminescence and Application to the Determination of Biological Reductants Using Lucigenin

Willie L. Hinze, Terrence E. Riehl, H. N. Singh, Yoshimi Baba

Research output: Contribution to journalArticle

99 Scopus citations

Abstract

The feasibility of using aqueous surfactant micelles to Improve analytical chemiluminescence (CL) measurements was examined. Specifically, the luclgenin (Luc)-N-methylacrldone CL system was characterized in different micellar and homogeneous media and the effects of various micellar systems upon the CL determination of biological reductants using Luc were assessed. It was found that solubility problems are obviated If the Luc CL assay is conducted In aqueous micelles. Additionally, the presence of cationic or zwltterionlc micelles Improves the sensitivity of the Luc CL assays for ascorbic acid, uric acid, glucose, and fructose by factors of 1.8-4.0. The average precision of the measurements Is ~9% In the micellar media while the accuracy Is comparable to that obtained In water alone. In a few Instances, potential problems due to mutual interference from other reductants can be eliminated via use of micelles. A brief prospective on the general applicability of micellar-enhanced CL In chemical analysis Is given.

Original languageEnglish
Pages (from-to)2180-2191
Number of pages12
JournalAnalytical Chemistry
Volume56
Issue number12
DOIs
StatePublished - Oct 1984

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