Methods to study pulmonary dendritic cell migration.

Claudia Jakubzick, Gwendalyn J. Randolph

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Dendritic cell migration from the airway to the lymph nodes is a key event in the development of airway immunity during infection, allergy, and vaccination. With judicial selection of materials, there are two approaches to study dendritic cell migration to the mediastinal lymph nodes without the induction of inflammation: airway administration of fluorescent OVA and latex beads. Our protocol describes how to label and track pulmonary dendritic cells from the airway and lung to the mediastinal lymph nodes and reveals how to avoid pitfalls and suboptimal assays.

Original languageEnglish
Pages (from-to)371-382
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume595
StatePublished - 2010
Externally publishedYes

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