TY - JOUR
T1 - Methods to study pulmonary dendritic cell migration.
AU - Jakubzick, Claudia
AU - Randolph, Gwendalyn J.
PY - 2010
Y1 - 2010
N2 - Dendritic cell migration from the airway to the lymph nodes is a key event in the development of airway immunity during infection, allergy, and vaccination. With judicial selection of materials, there are two approaches to study dendritic cell migration to the mediastinal lymph nodes without the induction of inflammation: airway administration of fluorescent OVA and latex beads. Our protocol describes how to label and track pulmonary dendritic cells from the airway and lung to the mediastinal lymph nodes and reveals how to avoid pitfalls and suboptimal assays.
AB - Dendritic cell migration from the airway to the lymph nodes is a key event in the development of airway immunity during infection, allergy, and vaccination. With judicial selection of materials, there are two approaches to study dendritic cell migration to the mediastinal lymph nodes without the induction of inflammation: airway administration of fluorescent OVA and latex beads. Our protocol describes how to label and track pulmonary dendritic cells from the airway and lung to the mediastinal lymph nodes and reveals how to avoid pitfalls and suboptimal assays.
UR - http://www.scopus.com/inward/record.url?scp=75549088291&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-421-0_24
DO - 10.1007/978-1-60761-421-0_24
M3 - Article
C2 - 19941125
AN - SCOPUS:75549088291
SN - 1064-3745
VL - 595
SP - 371
EP - 382
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -