Method for the simultaneous quantitation of apolipoprotein E isoforms using tandem mass spectrometry

Kristin R. Wildsmith, Bomie Han, Randall J. Bateman

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Using apolipoprotein E (ApoE) as a model protein, we developed a protein isoform analysis method utilizing stable isotope labeling tandem mass spectrometry (SILT MS). ApoE isoforms are quantitated using the intensities of the b and y ions of the 13C-labeled tryptic isoform-specific peptides versus unlabeled tryptic isoform-specific peptides. The ApoE protein isoform analysis using SILT allows for the simultaneous detection and relative quantitation of different ApoE isoforms from the same sample. This method provides a less biased assessment of ApoE isoforms compared to antibody-dependent methods, and may lead to a better understanding of the biological differences between isoforms.

Original languageEnglish
Pages (from-to)116-118
Number of pages3
JournalAnalytical Biochemistry
Volume395
Issue number1
DOIs
StatePublished - Dec 1 2009
Externally publishedYes

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