TY - JOUR
T1 - Method for the simultaneous quantitation of apolipoprotein E isoforms using tandem mass spectrometry
AU - Wildsmith, Kristin R.
AU - Han, Bomie
AU - Bateman, Randall J.
PY - 2009/12/1
Y1 - 2009/12/1
N2 - Using apolipoprotein E (ApoE) as a model protein, we developed a protein isoform analysis method utilizing stable isotope labeling tandem mass spectrometry (SILT MS). ApoE isoforms are quantitated using the intensities of the b and y ions of the 13C-labeled tryptic isoform-specific peptides versus unlabeled tryptic isoform-specific peptides. The ApoE protein isoform analysis using SILT allows for the simultaneous detection and relative quantitation of different ApoE isoforms from the same sample. This method provides a less biased assessment of ApoE isoforms compared to antibody-dependent methods, and may lead to a better understanding of the biological differences between isoforms.
AB - Using apolipoprotein E (ApoE) as a model protein, we developed a protein isoform analysis method utilizing stable isotope labeling tandem mass spectrometry (SILT MS). ApoE isoforms are quantitated using the intensities of the b and y ions of the 13C-labeled tryptic isoform-specific peptides versus unlabeled tryptic isoform-specific peptides. The ApoE protein isoform analysis using SILT allows for the simultaneous detection and relative quantitation of different ApoE isoforms from the same sample. This method provides a less biased assessment of ApoE isoforms compared to antibody-dependent methods, and may lead to a better understanding of the biological differences between isoforms.
UR - http://www.scopus.com/inward/record.url?scp=70349154194&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2009.07.049
DO - 10.1016/j.ab.2009.07.049
M3 - Article
C2 - 19653990
AN - SCOPUS:70349154194
SN - 0003-2697
VL - 395
SP - 116
EP - 118
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -