Method for determination of specific activity of proteins in polyacrylamide gels

David H. Alpers; Robert Glickman

doi:10.1016/0003-2697(70)90190-9

Method for determination of specific activity of proteins in polyacrylamide gels

David H. Alpers
, Robert Glickman

Division of Gastroenterology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A method is described for estimating protein content and radioactivity from the same solution of dissolved acrylamide gel. The gels are made utilizing ethylene diacrylate as a cross-linking agent, which permits the gel to be dissolved in 1 N NaOH. Protein is then determined by standard methods and NCS is used as a solubilizer for liquid scintilation.

Original language	English
Pages (from-to)	314-320
Number of pages	7
Journal	Analytical Biochemistry
Volume	35
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(70)90190-9
State	Published - Jun 1970

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title = "Method for determination of specific activity of proteins in polyacrylamide gels",

abstract = "A method is described for estimating protein content and radioactivity from the same solution of dissolved acrylamide gel. The gels are made utilizing ethylene diacrylate as a cross-linking agent, which permits the gel to be dissolved in 1 N NaOH. Protein is then determined by standard methods and NCS is used as a solubilizer for liquid scintilation.",

author = "Alpers, \{David H.\} and Robert Glickman",

note = "Funding Information: ACKNOWLEDGMENTS These studies were supported in part by grants AM-04501 and AM-05280 from thn National Institutes of Health. We are indebted to Mrs. Gladys Drummey for excellent technical assistance and to Dr. Kurt J. Isselbacher, Department of Medicine, Harvard Medical School, for many helpful discussions.",

year = "1970",

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doi = "10.1016/0003-2697(70)90190-9",

language = "English",

volume = "35",

pages = "314--320",

journal = "Analytical Biochemistry",

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T1 - Method for determination of specific activity of proteins in polyacrylamide gels

AU - Alpers, David H.

AU - Glickman, Robert

N1 - Funding Information: ACKNOWLEDGMENTS These studies were supported in part by grants AM-04501 and AM-05280 from thn National Institutes of Health. We are indebted to Mrs. Gladys Drummey for excellent technical assistance and to Dr. Kurt J. Isselbacher, Department of Medicine, Harvard Medical School, for many helpful discussions.

PY - 1970/6

Y1 - 1970/6

N2 - A method is described for estimating protein content and radioactivity from the same solution of dissolved acrylamide gel. The gels are made utilizing ethylene diacrylate as a cross-linking agent, which permits the gel to be dissolved in 1 N NaOH. Protein is then determined by standard methods and NCS is used as a solubilizer for liquid scintilation.

AB - A method is described for estimating protein content and radioactivity from the same solution of dissolved acrylamide gel. The gels are made utilizing ethylene diacrylate as a cross-linking agent, which permits the gel to be dissolved in 1 N NaOH. Protein is then determined by standard methods and NCS is used as a solubilizer for liquid scintilation.

UR - https://www.scopus.com/pages/publications/0014802495

U2 - 10.1016/0003-2697(70)90190-9

DO - 10.1016/0003-2697(70)90190-9

M3 - Article

C2 - 5450426

AN - SCOPUS:0014802495

SN - 0003-2697

VL - 35

SP - 314

EP - 320

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Method for determination of specific activity of proteins in polyacrylamide gels

Abstract

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