TY - JOUR
T1 - Method for determination of specific activity of proteins in polyacrylamide gels
AU - Alpers, David H.
AU - Glickman, Robert
N1 - Funding Information:
ACKNOWLEDGMENTS These studies were supported in part by grants AM-04501 and AM-05280 from thn National Institutes of Health. We are indebted to Mrs. Gladys Drummey for excellent technical assistance and to Dr. Kurt J. Isselbacher, Department of Medicine, Harvard Medical School, for many helpful discussions.
PY - 1970/6
Y1 - 1970/6
N2 - A method is described for estimating protein content and radioactivity from the same solution of dissolved acrylamide gel. The gels are made utilizing ethylene diacrylate as a cross-linking agent, which permits the gel to be dissolved in 1 N NaOH. Protein is then determined by standard methods and NCS is used as a solubilizer for liquid scintilation.
AB - A method is described for estimating protein content and radioactivity from the same solution of dissolved acrylamide gel. The gels are made utilizing ethylene diacrylate as a cross-linking agent, which permits the gel to be dissolved in 1 N NaOH. Protein is then determined by standard methods and NCS is used as a solubilizer for liquid scintilation.
UR - http://www.scopus.com/inward/record.url?scp=0014802495&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(70)90190-9
DO - 10.1016/0003-2697(70)90190-9
M3 - Article
C2 - 5450426
AN - SCOPUS:0014802495
SN - 0003-2697
VL - 35
SP - 314
EP - 320
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -