Method for determination of specific activity of proteins in polyacrylamide gels

David H. Alpers, Robert Glickman

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A method is described for estimating protein content and radioactivity from the same solution of dissolved acrylamide gel. The gels are made utilizing ethylene diacrylate as a cross-linking agent, which permits the gel to be dissolved in 1 N NaOH. Protein is then determined by standard methods and NCS is used as a solubilizer for liquid scintilation.

Original languageEnglish
Pages (from-to)314-320
Number of pages7
JournalAnalytical Biochemistry
Volume35
Issue number2
DOIs
StatePublished - Jun 1970

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