Metal-Organic Framework as a Protective Coating for Biodiagnostic Chips

Congzhou Wang, Sirimuvva Tadepalli, Jingyi Luan, Keng Ku Liu, Jeremiah J. Morrissey, Evan D. Kharasch, Rajesh R. Naik, Srikanth Singamaneni

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

An experiment was conducted to demonstrate that metal-organic frameworks (MOFs) zeolitic imidazolate framework-8 (ZIF-8) can be used as a protective material to preserve the recognition capability of antibodies on biosensor surfaces stored at ambient and elevated temperatures. With the protection of the MOF, both rabbit IgG and anti-neutrophil gelatinase associated Lipocalin (NGAL) appended to plasmonic biosensors retained over 70% of recognition capability compared with complete loss in unprotected samples after one week of storage at room temperature, 40 and 60°C. Such antibody-based biosensors with enhanced thermal stability eliminate the need for a cold chain system during transportation and storage in an energy-efficient and environmentally-friendly fashion. Furthermore, the biofunctionality of the MOF-coated biochip can be restored by a simple water rinsing step, making it highly convenient for use in POC and resource-limited settings such as in an ambulance, intensive care unit, emergency room, battlefield, or the developing world.

Original languageEnglish
Article number1604433
JournalAdvanced Materials
Volume29
Issue number7
DOIs
StatePublished - Feb 2017

Keywords

  • ZIF-8 preservation
  • antibody
  • biodiagnostics
  • metal-organic framework
  • plasmonic biosensors

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