TY - JOUR
T1 - Metal-Organic Framework as a Protective Coating for Biodiagnostic Chips
AU - Wang, Congzhou
AU - Tadepalli, Sirimuvva
AU - Luan, Jingyi
AU - Liu, Keng Ku
AU - Morrissey, Jeremiah J.
AU - Kharasch, Evan D.
AU - Naik, Rajesh R.
AU - Singamaneni, Srikanth
N1 - Funding Information:
The authors acknowledge support from the Air Force Office of Scientific Research (Grant Nos. FA9550-15-1-0228 and 12RX11COR), AFRL/711 HPW, and the National Institutes of Health (Grant Nos. R21DK100759 and R01 CA141521). The authors thank the Nano Research Facility (NRF) at Washington University for providing access to electron microscopy facilities. The authors thank Mr. Qisheng Jiang forhe lp with TEM imaging.
PY - 2017/2
Y1 - 2017/2
N2 - An experiment was conducted to demonstrate that metal-organic frameworks (MOFs) zeolitic imidazolate framework-8 (ZIF-8) can be used as a protective material to preserve the recognition capability of antibodies on biosensor surfaces stored at ambient and elevated temperatures. With the protection of the MOF, both rabbit IgG and anti-neutrophil gelatinase associated Lipocalin (NGAL) appended to plasmonic biosensors retained over 70% of recognition capability compared with complete loss in unprotected samples after one week of storage at room temperature, 40 and 60°C. Such antibody-based biosensors with enhanced thermal stability eliminate the need for a cold chain system during transportation and storage in an energy-efficient and environmentally-friendly fashion. Furthermore, the biofunctionality of the MOF-coated biochip can be restored by a simple water rinsing step, making it highly convenient for use in POC and resource-limited settings such as in an ambulance, intensive care unit, emergency room, battlefield, or the developing world.
AB - An experiment was conducted to demonstrate that metal-organic frameworks (MOFs) zeolitic imidazolate framework-8 (ZIF-8) can be used as a protective material to preserve the recognition capability of antibodies on biosensor surfaces stored at ambient and elevated temperatures. With the protection of the MOF, both rabbit IgG and anti-neutrophil gelatinase associated Lipocalin (NGAL) appended to plasmonic biosensors retained over 70% of recognition capability compared with complete loss in unprotected samples after one week of storage at room temperature, 40 and 60°C. Such antibody-based biosensors with enhanced thermal stability eliminate the need for a cold chain system during transportation and storage in an energy-efficient and environmentally-friendly fashion. Furthermore, the biofunctionality of the MOF-coated biochip can be restored by a simple water rinsing step, making it highly convenient for use in POC and resource-limited settings such as in an ambulance, intensive care unit, emergency room, battlefield, or the developing world.
KW - ZIF-8 preservation
KW - antibody
KW - biodiagnostics
KW - metal-organic framework
KW - plasmonic biosensors
UR - http://www.scopus.com/inward/record.url?scp=85008264691&partnerID=8YFLogxK
U2 - 10.1002/adma.201604433
DO - 10.1002/adma.201604433
M3 - Article
C2 - 27925296
AN - SCOPUS:85008264691
VL - 29
JO - Advanced Materials
JF - Advanced Materials
SN - 0935-9648
IS - 7
M1 - 1604433
ER -