Metagenomic analysis of the human distal gut microbiome

Steven R. Gill, Mihai Pop, Robert T. DeBoy, Paul B. Eckburg, Peter J. Turnbaugh, Buck S. Samuel, Jeffrey I. Gordon, David A. Relman, Claire M. Fraser-Liggett, Karen E. Nelson

Research output: Contribution to journalArticle

2618 Scopus citations

Abstract

The human intestinal microbiota is composed of 1013 to 10 14 microorganisms whose collective genome ("microbiome") contains at least 100 times as many genes as our own genome. We analyzed ∼78 million base pairs of unique DNA sequence and 2062 polymerase chain reaction-amplified 165 ribosomal DNA sequences obtained from the fecal DNAs of two healthy adults. Using metabolic function analyses of identified genes, we compared our human genome with the average content of previously sequenced microbial genomes. Our microbiome has significantly enriched metabolism of glycans, amino acids, and xenobiotics; methanogenesis; and 2-methyl-D-erythritol 4-phosphate pathway-mediated biosynthesis of vitamins and isoprenoids. Thus, humans are superorganisms whose metabolism represents an amalgamation of microbial and human attributes.

Original languageEnglish
Pages (from-to)1355-1359
Number of pages5
JournalScience
Volume312
Issue number5778
DOIs
StatePublished - Jun 2 2006

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    Gill, S. R., Pop, M., DeBoy, R. T., Eckburg, P. B., Turnbaugh, P. J., Samuel, B. S., Gordon, J. I., Relman, D. A., Fraser-Liggett, C. M., & Nelson, K. E. (2006). Metagenomic analysis of the human distal gut microbiome. Science, 312(5778), 1355-1359. https://doi.org/10.1126/science.1124234