TY - JOUR
T1 - Metabolism of receptor targeted 111In-DTPA-glycoproteins
T2 - Identification of 111In-DTPA-ϵ-lysine as the primary metabolic and excretory product
AU - Franano, F. Nicholas
AU - Edwards, W. Barry
AU - Welch, Michael J.
AU - Duncan, James R.
PY - 1994/11
Y1 - 1994/11
N2 - The hepatic and renal retention of indium-111 (111In) from 111In-labeled polypeptides has been the subject of many investigations. Because the lysosome is a common intracellular destination for the degradation of polypeptides, we studied the lysosomal metabolism of 111In-DTPA-labeled glycoproteins targeted to cell surface receptors in vitro and in vivo. We found that 111In-DTPA-glycoproteins were degraded to 111In-DTPA-ε{lunate}-lysine, which was slowly released from cells and recovered intact in urine and feces. These results suggest a mechanism for 111In retention at target and non-target sites.
AB - The hepatic and renal retention of indium-111 (111In) from 111In-labeled polypeptides has been the subject of many investigations. Because the lysosome is a common intracellular destination for the degradation of polypeptides, we studied the lysosomal metabolism of 111In-DTPA-labeled glycoproteins targeted to cell surface receptors in vitro and in vivo. We found that 111In-DTPA-glycoproteins were degraded to 111In-DTPA-ε{lunate}-lysine, which was slowly released from cells and recovered intact in urine and feces. These results suggest a mechanism for 111In retention at target and non-target sites.
UR - http://www.scopus.com/inward/record.url?scp=0028631160&partnerID=8YFLogxK
U2 - 10.1016/0969-8051(94)90174-0
DO - 10.1016/0969-8051(94)90174-0
M3 - Article
C2 - 9234360
AN - SCOPUS:0028631160
SN - 0969-8051
VL - 21
SP - 1023
EP - 1034
JO - Nuclear Medicine and Biology
JF - Nuclear Medicine and Biology
IS - 8
ER -