Metabolism of oxygenated derivatives of arachidonic acid by Caco-2 cells

T. E. Riehl, J. Turk, W. F. Stenson

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Monolayers of Caco-2 cells, a human enterocyte cell line, were incubated separately with 3H8-labeled preparations of three different lipid mediators of inflammation: 5-hydroxyeicosatetraenoic acid, 12-hydroxyeicosatetraenoic acid, and leukotriene B4. Both [3H8]5-hydroxyeicosatetraenoic and [3H8]12-hydroxyeicosatetraenoic acids were taken up and metabolized by Caco-2 cells, but [3H]leukotriene B4 remained unmetabolized in the incubation medium. [3H]5-hydroxyeicosatetraenoic acid was esterified into cellular phospholipids (15%) and triglycerides (4%) but did not undergo β- oxidation. When [3H]12-hydroxyeicosatetraenoic acid was incubated with Caco- 2 cells, 14% underwent two cycles of β-oxidation to form [3H]8- hydroxyhexadecatrienoic acid, and 3% underwent three cycles of β-oxidation to form [3H]6-hydroxytetradecadienoic acid, both of which were released into the media. [3H]12-Hydroxyeicosatetraenoic acid was also esterified into cellular phospholipids (13%), but none was esterified into cellular triglycerides.

Original languageEnglish
Pages (from-to)323-331
Number of pages9
JournalJournal of lipid research
Volume33
Issue number3
StatePublished - 1992

Keywords

  • 12-HETE
  • 15-HETE
  • 5-HETE
  • 6- hydroxytetradecadienoic acid
  • 8-hydroxyhexadecatrienoic acid
  • LTB
  • inflammatory bowel disease

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