Metabolism of glyophosate in Pseudomonas sp. strain LBr

G. S. Jacob, J. R. Garbow, L. E. Hallas, N. M. Kimack, G. M. Kishore, J. Schaeffer

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97 Scopus citations

Abstract

Metabolism of glyophosate (N-phosphonomethylglycine) by Pseudomonas sp. strain LBr, a bacterium isolated from a glyphosate process waste stream, was examined by a combination of solid-state 13C nuclear magnetic resonance experiments and analysis of the phosphonate composition of the growth medium. Pseudomonas sp. strain LBr was capable of eliminating 20 mM glyphosate from the growth medium, an amount approximately 20-fold greater than that reported for any other microorganism to date. The bacterium degraded high levels of glyphosate, primarily by converting it to aminomethylphosphonate, followed by release into the growth medium. Only a small amount of aminomethylphosphonate (about 0.5 to 0.7 mM), which is needed to supply phosphorus for growth, could be metabolized by the microorganism. Solid-state 13C nuclear magnetic resonance analysis of strain LBr grown on 1 mM [2-13C,15N]glyphosate showed that about 5% of the glyphosate was degraded by a separate pathway involving breakdown of glyphosate to glycine, a pathway first observed in Pseudomonas sp. strain PG2982. Thus, Pseudomonas sp. strain LBr appears to possess two distinct routes for glyphosate detoxification.

Original languageEnglish
Pages (from-to)2953-2958
Number of pages6
JournalApplied and Environmental Microbiology
Volume54
Issue number12
StatePublished - Jan 1 1988
Externally publishedYes

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    Jacob, G. S., Garbow, J. R., Hallas, L. E., Kimack, N. M., Kishore, G. M., & Schaeffer, J. (1988). Metabolism of glyophosate in Pseudomonas sp. strain LBr. Applied and Environmental Microbiology, 54(12), 2953-2958.