TY - JOUR
T1 - Metabolism in vitro of radioiodinated N-isopropyl-p-iodoamphetamine by isolated hepatocytes
AU - Moerlein, Stephen M.
AU - Weisman, Robert A.
AU - Beck, Dale
AU - Li, Albert P.
AU - Welch, Michael J.
PY - 1993/1
Y1 - 1993/1
N2 - An in vitro technique for the determination of radiopharmaceutical metabolism has been developed using isolated hepatocytes. Radioiodinated N-isopropyl-p-iodoamphetamine (IMP; iofetamine, USP) was employed a model tracer in these studies because its labeled metabolites are well-characterized. Hepatocytes isolated from the rat and human produced labeled metabolites in vitro for up to 4h in a manner similar to that reported for humans in vivo. Identical metabolites were generated by all cell types investigated, but the rate of metabolism differed (rat ≫ human; female > male; fresh > frozen). The utility of this methodology for the preclinical evaluation of potential radiopharmaceuticals is described.
AB - An in vitro technique for the determination of radiopharmaceutical metabolism has been developed using isolated hepatocytes. Radioiodinated N-isopropyl-p-iodoamphetamine (IMP; iofetamine, USP) was employed a model tracer in these studies because its labeled metabolites are well-characterized. Hepatocytes isolated from the rat and human produced labeled metabolites in vitro for up to 4h in a manner similar to that reported for humans in vivo. Identical metabolites were generated by all cell types investigated, but the rate of metabolism differed (rat ≫ human; female > male; fresh > frozen). The utility of this methodology for the preclinical evaluation of potential radiopharmaceuticals is described.
UR - http://www.scopus.com/inward/record.url?scp=0027393080&partnerID=8YFLogxK
U2 - 10.1016/0969-8051(93)90135-H
DO - 10.1016/0969-8051(93)90135-H
M3 - Article
C2 - 8461879
AN - SCOPUS:0027393080
SN - 0969-8051
VL - 20
SP - 49
EP - 56
JO - Nuclear Medicine and Biology
JF - Nuclear Medicine and Biology
IS - 1
ER -