TY - JOUR
T1 - Metabolic responses of osteochondral allografts to re-warming
AU - Stoker, Aaron M.
AU - Caldwell, Katherine M.
AU - Stannard, James P.
AU - Cook, James L.
N1 - Publisher Copyright:
© 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
PY - 2019/7
Y1 - 2019/7
N2 - Symptomatic chondral and osteochondral defects affect a large and growing number of patients. A safe and effective surgical treatment for large articular defects is osteochondral allograft (OCA) transplantation. One of the major causes of failure for OCA transplantation is loss of essential chondrocyte viability during the preservation and storage period. It is also possible that metabolic responses of the OCA when transitioning from storage temperature to body temperature may contribute to mechanisms causing failure. The present study was designed to compare MOPSSM-preserved OCAs to those stored using the current standard of care (SOC) method with respect to metabolic responses when rewarmed for transplantation to and maintenance at body temperature (37°C). It was theorized that grafts stored using the MOPSSM protocol would maintain significantly higher chondrocyte viability and produce significantly lower levels of inflammatory mediators and degradative enzymes, and significantly higher levels of chemokines compared to grafts stored using the SOC protocol. Left over SOC and MOPSSM-stored OCA tissues were collected after surgery, and cartilage explants were cultured for 6 days. Media was analyzed for biomarkers using commercially available assays. Cartilage from SOC grafts released significantly higher levels of PGE2, MMP-1, MMP-2, and MMP-13, and significantly lower levels of IL-8 and Gro-α, compared to cartilage from MOPSSM-stored grafts. Clinical significance: These data suggest that OCAs stored using the MOPSSM protocol have potentially less detrimental initial inflammatory and degradative responses to re-warming for transplantation compared to OCAs stored using the current tissue bank protocols.
AB - Symptomatic chondral and osteochondral defects affect a large and growing number of patients. A safe and effective surgical treatment for large articular defects is osteochondral allograft (OCA) transplantation. One of the major causes of failure for OCA transplantation is loss of essential chondrocyte viability during the preservation and storage period. It is also possible that metabolic responses of the OCA when transitioning from storage temperature to body temperature may contribute to mechanisms causing failure. The present study was designed to compare MOPSSM-preserved OCAs to those stored using the current standard of care (SOC) method with respect to metabolic responses when rewarmed for transplantation to and maintenance at body temperature (37°C). It was theorized that grafts stored using the MOPSSM protocol would maintain significantly higher chondrocyte viability and produce significantly lower levels of inflammatory mediators and degradative enzymes, and significantly higher levels of chemokines compared to grafts stored using the SOC protocol. Left over SOC and MOPSSM-stored OCA tissues were collected after surgery, and cartilage explants were cultured for 6 days. Media was analyzed for biomarkers using commercially available assays. Cartilage from SOC grafts released significantly higher levels of PGE2, MMP-1, MMP-2, and MMP-13, and significantly lower levels of IL-8 and Gro-α, compared to cartilage from MOPSSM-stored grafts. Clinical significance: These data suggest that OCAs stored using the MOPSSM protocol have potentially less detrimental initial inflammatory and degradative responses to re-warming for transplantation compared to OCAs stored using the current tissue bank protocols.
KW - chondrocyte viability
KW - osteochondral allografts
KW - re-warming
KW - tissue preservation methods
KW - transplantation
UR - http://www.scopus.com/inward/record.url?scp=85063563292&partnerID=8YFLogxK
U2 - 10.1002/jor.24290
DO - 10.1002/jor.24290
M3 - Article
C2 - 30912859
AN - SCOPUS:85063563292
SN - 0736-0266
VL - 37
SP - 1530
EP - 1536
JO - Journal of Orthopaedic Research
JF - Journal of Orthopaedic Research
IS - 7
ER -