Metabolic niche of a prominent sulfate-reducing human gut bacterium

Federico E. Rey, Mark D. Gonzalez, Jiye Cheng, Meng Wu, Philip P. Ahern, Jeffrey I. Gordon

Research output: Contribution to journalArticle

163 Scopus citations

Abstract

Sulfate-reducing bacteria (SRB) colonize the guts of ?50% of humans. We used genome-wide transposon mutagenesis and insertion-site sequencing, RNA-Seq, plus mass spectrometry to characterize genetic and environmental factors that impact the niche of Desulfovibrio piger, the most common SRB in a surveyed cohort of healthy US adults. Gnotobiotic mice were colonized with an assemblage of sequenced human gut bacterial species with or without D. piger and fed diets with different levels and types of carbohydrates and sulfur sources. Diet was a major determinant of functions expressed by this artificial nine-member community and of the genes that impact D. piger fitness; the latter includes high-and low-affinity systems for using ammonia, a limiting resource for D. piger in mice consuming a polysaccharide-rich diet. Although genes involved in hydrogen consumption and sulfate reduction are necessary for its colonization, varying dietary-free sulfate levels did not significantly alter levels of D. piger, which can obtain sulfate from the host in part via cross-feeding mediated by Bacteroides-encoded sulfatases. Chondroitin sulfate, a common dietary supplement, increased D. piger and H2S levels without compromising gut barrier integrity. A chondroitin sulfate-supplemented diet together with D. piger impacted the assemblage's substrate utilization preferences, allowing consumption of more reduced carbon sources and increasing the abundance of the H2-producing Actinobacterium, Collinsella aerofaciens. Our findings provide genetic and metabolic details of how this H2-consuming SRB shapes the responses of a microbiota to diet ingredients and a framework for examining how individuals lacking D. piger differ from those who harbor it.

Original languageEnglish
Pages (from-to)13582-13587
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number33
DOIs
StatePublished - Aug 13 2013

Keywords

  • Artificial human gut microbiota/microbiome
  • Determinants of microbial fitness
  • Hydrogen sulfide
  • Hydrogenotrophs
  • Microbial foodwebs

Fingerprint Dive into the research topics of 'Metabolic niche of a prominent sulfate-reducing human gut bacterium'. Together they form a unique fingerprint.

  • Cite this