TY - JOUR
T1 - Metabolic importance of adipose tissue monoacylglycerol acyltransferase 1 in mice and humans
AU - Liss, Kim H.H.
AU - Lutkewitte, Andrew J.
AU - Pietka, Terri
AU - Finck, Brian N.
AU - Franczyk, Michael
AU - Yoshino, Jun
AU - Klein, Samuel
AU - Hall, Angela M.
N1 - Funding Information:
This work was funded by the American Diabetes Association Grant 17-IBS-109 (A.M.H.) and the National Institute of Diabetes and Digestive and Kidney Diseases Grants R56 DK111735 (A.M.H.) R01 DK078187 (B.N.F.), and R01 DK104995 (J.Y.). as well as a grant from the Pershing Square Foundation (S.K.). The Core services of the Diabetes Research Center (P30 DK020579) and the Nutrition Obesity Research Center (P30 DK56341) at the Washington University School of Medicine also supported this work. K.H.H.L is a Pediatric Gastroenterology Training Grant Fellow supported by T32 DK0077653. A.J.L. is supported by the Diabetes Research Postdoctoral Training Program fellowship (T32 DK007120). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Funding Information:
This work was funded by the American Diabetes Association Grant 17-IBS-109 (A.M.H.) and the National Institute of Diabetes and Digestive and Kidney Diseases Grants R56 DK111735 (A.M.H.) R01 DK078187 (B.N.F.), and R01 DK104995 (J.Y.). as well as a grant from the Pershing Square Foundation (S.K.). The Core services of the Diabetes Research Center (P30 DK020579) and the Nutrition Obesity Research Center (P30 DK56341) at the Washington University School of Medicine also supported this work. K.H.H.L is a Pediatric Gastroenterology Training Grant Fellow supported by T32 DK0077653. A.J.L. is supported by the Diabetes Research Postdoctoral Training Program fellowship (T32 DK007120). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Author’s Choice—Final version open access under the terms of the Creative Commons CC-BY license. Manuscript received 8 March 2018 and in revised form 10 May 2018. Published, JLR Papers in Press, May 31, 2018 DOI https://doi.org/10.1194/jlr.M084947
Publisher Copyright:
Copyright © 2018 Liss et al. Published by The American Society for Biochemistry and Molecular Biology, Inc.
PY - 2018
Y1 - 2018
N2 - Adipocyte triglyceride storage provides a reservoir of energy that allows the organism to survive times of nutrient scarcity, but excessive adiposity has emerged as a health problem in many areas of the world. Monoacylglyc-erol acyltransferase (MGAT) acylates monoacylglycerol to produce diacylglycerol; the penultimate step in triglyceride synthesis. However, little is known about MGAT activity in adipocytes, which are believed to rely primarily on another pathway for triglyceride synthesis. We show that expression of the gene that encodes MGAT1 is robustly induced during adipocyte differentiation and that its expression is suppressed in fat of genetically-obese mice and metabolically-abnormal obese human subjects. Interestingly, MGAT1 expression is also reduced in physiologic contexts where lipolysis is high. Moreover, knockdown or knockout of MGAT1 in adipocytes leads to higher rates of basal adipocyte lipolysis. Collectively, these data suggest that MGAT1 activity may play a role in regulating basal adipocyte FFA retention.—Liss, K. H. H., A. J. Lutkewitte, T. Pietka, B. N. Finck, M. Franczyk, J. Yoshino, S. Klein, and A. M. Hall. Metabolic importance of adipose tissue monoacylglycerol acyltransferase 1 in mice and humans.
AB - Adipocyte triglyceride storage provides a reservoir of energy that allows the organism to survive times of nutrient scarcity, but excessive adiposity has emerged as a health problem in many areas of the world. Monoacylglyc-erol acyltransferase (MGAT) acylates monoacylglycerol to produce diacylglycerol; the penultimate step in triglyceride synthesis. However, little is known about MGAT activity in adipocytes, which are believed to rely primarily on another pathway for triglyceride synthesis. We show that expression of the gene that encodes MGAT1 is robustly induced during adipocyte differentiation and that its expression is suppressed in fat of genetically-obese mice and metabolically-abnormal obese human subjects. Interestingly, MGAT1 expression is also reduced in physiologic contexts where lipolysis is high. Moreover, knockdown or knockout of MGAT1 in adipocytes leads to higher rates of basal adipocyte lipolysis. Collectively, these data suggest that MGAT1 activity may play a role in regulating basal adipocyte FFA retention.—Liss, K. H. H., A. J. Lutkewitte, T. Pietka, B. N. Finck, M. Franczyk, J. Yoshino, S. Klein, and A. M. Hall. Metabolic importance of adipose tissue monoacylglycerol acyltransferase 1 in mice and humans.
UR - http://www.scopus.com/inward/record.url?scp=85052660917&partnerID=8YFLogxK
U2 - 10.1194/jlr.M084947
DO - 10.1194/jlr.M084947
M3 - Article
C2 - 29853530
AN - SCOPUS:85052660917
SN - 0022-2275
VL - 59
SP - 1630
EP - 1639
JO - Journal of Lipid Research
JF - Journal of Lipid Research
IS - 9
ER -