Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs

R. Coleman Lindsley, Jennifer G. Gill, Theresa L. Murphy, Ellen M. Langer, Mi Cai, Mona Mashayekhi, Wei Wang, Noriko Niwa, Jeanne M. Nerbonne, Michael Kyba, Kenneth M. Murphy

Research output: Contribution to journalArticle

143 Scopus citations

Abstract

Wnt signaling is required for development of mesoderm- derived lineages and expression of transcription factors associated with the primitive streak. In a functional screen, we examined the mesoderminducing capacity of transcription factors whose expression was Wnt-dependent in differentiating ESCs. In contrast to many inactive factors, we found that mesoderm posterior 1 (Mesp1) promoted mesoderm development independently of Wnt signaling. Transient Mesp1 expression in ESCs promotes changes associated with epithelial-mesenchymal transition (EMT) and induction of Snai1, consistent with a role in gastrulation. Mesp1 expression also restricted the potential fates derived from ESCs, generating mesoderm progenitors with cardiovascular, but not hematopoietic, potential. Thus, in addition to its effects on EMT, Mesp1 may be capable of generating the recently identified multipotent cardiovascular progenitor from ESCs in vitro.

Original languageEnglish
Pages (from-to)55-68
Number of pages14
JournalCell Stem Cell
Volume3
Issue number1
DOIs
StatePublished - Jul 3 2008

Fingerprint Dive into the research topics of 'Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs'. Together they form a unique fingerprint.

  • Cite this