Mesothelin is a specific biomarker of invasive cancer in the Barrett-associated adenocarcinoma progression model: translational implications for diagnosis and therapy

Hector Alvarez, Pamela Leal Rojas, Ken Tye Yong, Hong Ding, Gaixia Xu, Paras N. Prasad, Jean Wang, Marcia Canto, James R. Eshleman, Elizabeth A. Montgomery, Anirban Maitra

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Esophageal adenocarcinoma arises in the backdrop of Barrett metaplasia-dysplasia sequence, with the vast majority of patients presenting with late-stage malignancy. Mesothelin, a glycophosphatidylinositol-anchored protein, is aberrantly overexpressed on the surface of many solid cancers. Mesothelin expression was assessed in esophageal tissue microarrays encompassing the entire histological spectrum of Barrett-associated dysplasia and adenocarcinoma. Mesothelin expression was observed in 24/84 (29%) of invasive adenocarcinomas and in 5/34 (15%) lymph node metastases. In contrast, normal squamous and cardiac mucosa, as well as noninvasive Barrett lesions, failed to label with mesothelin. Mesothelin was expressed in the esophageal adenocarcinoma cell line JH-EsoAd1 but not in primary human esophageal epithelial cells. Anti-mesothelin antibody-conjugated CdSe/CDS/ZnS quantum rods were synthesized, and confocal bioimaging confirmed robust binding to JH-EsoAd1 cells. Anti-mesothelin antibody-conjugated nanoparticles can be useful for the diagnosis and therapy of mesothelin-overexpressing esophageal adenocarcinomas.

Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume4
Issue number4
DOIs
StatePublished - Dec 1 2008

Keywords

  • Antibody-conjugated nanoparticles
  • Barrett esophagus
  • Immunohistochemistry
  • Mesothelin
  • Molecular imaging
  • Quantum rods

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