Mendelian analysis of the organization of actin sequences in Physarum polycephalum

Tim Schedl, William F. Dove

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

The genormic organization of the multiple actin DNA sequences in the lower eukaryote Physarum polycephalum was investigated by Mendelian mapping. Actin-homologous restriction endonuclease cleavage fragments detected by DNA blotting showed length polymorphisms when different strains were compared. These length polymorphisms were used as phenotypic markers for actin sequences in the genome. The meiotic assortment of the polymorphic restriction fragments was analysed, revealing four unlinked actin loci. The data for three-of the actin loci, ardB, C and D, are consistent with a single sequence or gene at each locus. The data for the other actin locus. ardA, is consistent with multiple linked actin sequences or genes.

Original languageEnglish
Pages (from-to)41-57
Number of pages17
JournalJournal of Molecular Biology
Volume160
Issue number1
DOIs
StatePublished - Sep 5 1982

Fingerprint

Dive into the research topics of 'Mendelian analysis of the organization of actin sequences in Physarum polycephalum'. Together they form a unique fingerprint.

Cite this