TY - JOUR
T1 - Mendelian analysis of the organization of actin sequences in Physarum polycephalum
AU - Schedl, Tim
AU - Dove, William F.
N1 - Funding Information:
This investigation was supported by Program-Project grant CA-23076 in Tumor Biology and Core grant CA-07175 to the McArdle Laboratory from the Sational Cancer Institute. This paper is dedicated to Norman Davidson on the occasion of his 65th birthday.
PY - 1982/9/5
Y1 - 1982/9/5
N2 - The genormic organization of the multiple actin DNA sequences in the lower eukaryote Physarum polycephalum was investigated by Mendelian mapping. Actin-homologous restriction endonuclease cleavage fragments detected by DNA blotting showed length polymorphisms when different strains were compared. These length polymorphisms were used as phenotypic markers for actin sequences in the genome. The meiotic assortment of the polymorphic restriction fragments was analysed, revealing four unlinked actin loci. The data for three-of the actin loci, ardB, C and D, are consistent with a single sequence or gene at each locus. The data for the other actin locus. ardA, is consistent with multiple linked actin sequences or genes.
AB - The genormic organization of the multiple actin DNA sequences in the lower eukaryote Physarum polycephalum was investigated by Mendelian mapping. Actin-homologous restriction endonuclease cleavage fragments detected by DNA blotting showed length polymorphisms when different strains were compared. These length polymorphisms were used as phenotypic markers for actin sequences in the genome. The meiotic assortment of the polymorphic restriction fragments was analysed, revealing four unlinked actin loci. The data for three-of the actin loci, ardB, C and D, are consistent with a single sequence or gene at each locus. The data for the other actin locus. ardA, is consistent with multiple linked actin sequences or genes.
UR - http://www.scopus.com/inward/record.url?scp=0020411349&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(82)90130-9
DO - 10.1016/0022-2836(82)90130-9
M3 - Article
C2 - 6294303
AN - SCOPUS:0020411349
SN - 0022-2836
VL - 160
SP - 41
EP - 57
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 1
ER -