Abstract
The effect of different concentrations (0.1-5 μM) of neurokinin B (NKB) and Aβ (25-35) on acetylcholine esterase (AChE), Na+-K+ ATPase and membrane fluidity (DPH anisotropy) were investigated in rat brain synaptosomes of 3, 9, 18 and 30 months old. An age dependent decrease was observed for all the three parameters studied. An in vitro incubation of isolated brain synaptosomes with Aβ (25-35) showed toxic effects on all the parameters studied and the peptide had concentration and age dependent effects, while NKB showed stimulating effect on the parameters and the combined NKB+Aβ (25-35) incubations showed a partial reversal effect as compared to the Aβ (25-35) alone. Thus, the results suggest a membrane mediated function for NKB and its role in neuromodulation, neuroprotection and antioxidant property against Aβ (25-35) induced toxicity in aging brain functions.
Original language | English |
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Pages (from-to) | 19-33 |
Number of pages | 15 |
Journal | Biogerontology |
Volume | 7 |
Issue number | 1 |
DOIs | |
State | Published - Feb 2006 |
Externally published | Yes |
Keywords
- Aging
- Amyloid beta protein
- Membrane dynamics
- Neurokinin B (NKB)
- Neuroprotection
- Oxidative stress and excitotoxicity
- Tachykinins