Membrane associated functions of neurokinin B (NKB) on Aβ (25-35) induced toxicity in aging rat brain synaptosomes

Anil K. Mantha; K. Moorthy; Sudha M. Cowsik; Najma Z. Baquer

doi:10.1007/s10522-005-6044-z

Membrane associated functions of neurokinin B (NKB) on Aβ (25-35) induced toxicity in aging rat brain synaptosomes

Anil K. Mantha, K. Moorthy, Sudha M. Cowsik, Najma Z. Baquer

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The effect of different concentrations (0.1-5 μM) of neurokinin B (NKB) and Aβ (25-35) on acetylcholine esterase (AChE), Na⁺-K⁺ ATPase and membrane fluidity (DPH anisotropy) were investigated in rat brain synaptosomes of 3, 9, 18 and 30 months old. An age dependent decrease was observed for all the three parameters studied. An in vitro incubation of isolated brain synaptosomes with Aβ (25-35) showed toxic effects on all the parameters studied and the peptide had concentration and age dependent effects, while NKB showed stimulating effect on the parameters and the combined NKB+Aβ (25-35) incubations showed a partial reversal effect as compared to the Aβ (25-35) alone. Thus, the results suggest a membrane mediated function for NKB and its role in neuromodulation, neuroprotection and antioxidant property against Aβ (25-35) induced toxicity in aging brain functions.

Original language	English
Pages (from-to)	19-33
Number of pages	15
Journal	Biogerontology
Volume	7
Issue number	1
DOIs	https://doi.org/10.1007/s10522-005-6044-z
State	Published - Feb 2006
Externally published	Yes

Keywords

Aging
Amyloid beta protein
Membrane dynamics
Neurokinin B (NKB)
Neuroprotection
Oxidative stress and excitotoxicity
Tachykinins

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title = "Membrane associated functions of neurokinin B (NKB) on Aβ (25-35) induced toxicity in aging rat brain synaptosomes",

abstract = "The effect of different concentrations (0.1-5 μM) of neurokinin B (NKB) and Aβ (25-35) on acetylcholine esterase (AChE), Na+-K+ ATPase and membrane fluidity (DPH anisotropy) were investigated in rat brain synaptosomes of 3, 9, 18 and 30 months old. An age dependent decrease was observed for all the three parameters studied. An in vitro incubation of isolated brain synaptosomes with Aβ (25-35) showed toxic effects on all the parameters studied and the peptide had concentration and age dependent effects, while NKB showed stimulating effect on the parameters and the combined NKB+Aβ (25-35) incubations showed a partial reversal effect as compared to the Aβ (25-35) alone. Thus, the results suggest a membrane mediated function for NKB and its role in neuromodulation, neuroprotection and antioxidant property against Aβ (25-35) induced toxicity in aging brain functions.",

keywords = "Aging, Amyloid beta protein, Membrane dynamics, Neurokinin B (NKB), Neuroprotection, Oxidative stress and excitotoxicity, Tachykinins",

author = "Mantha, {Anil K.} and K. Moorthy and Cowsik, {Sudha M.} and Baquer, {Najma Z.}",

note = "Funding Information: The authors acknowledge the financial support from UGC in the form the Excellence project awarded to Jawaharlal Nehru University. Anil K. Mantha a Senior Research Fellow was supported by Indian Council of Medical Research, New Delhi, India.",

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doi = "10.1007/s10522-005-6044-z",

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AU - Moorthy, K.

AU - Cowsik, Sudha M.

AU - Baquer, Najma Z.

N1 - Funding Information: The authors acknowledge the financial support from UGC in the form the Excellence project awarded to Jawaharlal Nehru University. Anil K. Mantha a Senior Research Fellow was supported by Indian Council of Medical Research, New Delhi, India.

PY - 2006/2

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N2 - The effect of different concentrations (0.1-5 μM) of neurokinin B (NKB) and Aβ (25-35) on acetylcholine esterase (AChE), Na+-K+ ATPase and membrane fluidity (DPH anisotropy) were investigated in rat brain synaptosomes of 3, 9, 18 and 30 months old. An age dependent decrease was observed for all the three parameters studied. An in vitro incubation of isolated brain synaptosomes with Aβ (25-35) showed toxic effects on all the parameters studied and the peptide had concentration and age dependent effects, while NKB showed stimulating effect on the parameters and the combined NKB+Aβ (25-35) incubations showed a partial reversal effect as compared to the Aβ (25-35) alone. Thus, the results suggest a membrane mediated function for NKB and its role in neuromodulation, neuroprotection and antioxidant property against Aβ (25-35) induced toxicity in aging brain functions.

AB - The effect of different concentrations (0.1-5 μM) of neurokinin B (NKB) and Aβ (25-35) on acetylcholine esterase (AChE), Na+-K+ ATPase and membrane fluidity (DPH anisotropy) were investigated in rat brain synaptosomes of 3, 9, 18 and 30 months old. An age dependent decrease was observed for all the three parameters studied. An in vitro incubation of isolated brain synaptosomes with Aβ (25-35) showed toxic effects on all the parameters studied and the peptide had concentration and age dependent effects, while NKB showed stimulating effect on the parameters and the combined NKB+Aβ (25-35) incubations showed a partial reversal effect as compared to the Aβ (25-35) alone. Thus, the results suggest a membrane mediated function for NKB and its role in neuromodulation, neuroprotection and antioxidant property against Aβ (25-35) induced toxicity in aging brain functions.

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KW - Amyloid beta protein

KW - Membrane dynamics

KW - Neurokinin B (NKB)

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KW - Oxidative stress and excitotoxicity

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Membrane associated functions of neurokinin B (NKB) on Aβ (25-35) induced toxicity in aging rat brain synaptosomes

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