Members of adenovirus species B utilize CD80 and CD86 as cellular attachment receptors

Joshua J. Short, Chenthamarakshan Vasu, Mark J. Holterman, David T. Curiel, Alexander Pereboev

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

Alternate serotypes of adenovirus (Ad), including Ads of species B, are being explored to circumvent the disadvantages of Ad serotype 5 gene delivery vectors. Whereas the majority of human Ads utilize the Coxsackievirus and adenovirus receptor (CAR), none of the Ad species B use CAR. Ad species B is further divided into two subspecies, B1 and B2, and utilizes at least two classes of receptors: common Ad species B receptors and B2 specific receptors. CD46 has been implicated as a B2-specific receptor. Ad serotype 3 (Ad3), a member of B1, utilizes CD80 and CD86 as cellular attachment receptors. The receptor-interacting Ad fiber-knob domain is highly homologous among species B Ads. We hypothesized that other members of Ad species B may utilize CD80 and CD86 as cellular attachment receptors. All tested species B members showed specific binding to cells expressing CD80 and CD86, and the Ad fiber-knob domain from both B1 and B2 Ad efficiently blocked CD80- and CD86-mediated infection of Ad3 vectors. Members of both B1 and B2 demonstrated CD80- and CD86-specific infection of CHO cells expressing CD80 and CD86. Therefore, all of the members of Ad species B utilize CD80 and CD86 for infection of cells.

Original languageEnglish
Pages (from-to)144-153
Number of pages10
JournalVirus Research
Volume122
Issue number1-2
DOIs
StatePublished - Dec 2006

Keywords

  • Adenovirus species B
  • CD80
  • CD86
  • Receptor

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