TY - JOUR
T1 - Members of adenovirus species B utilize CD80 and CD86 as cellular attachment receptors
AU - Short, Joshua J.
AU - Vasu, Chenthamarakshan
AU - Holterman, Mark J.
AU - Curiel, David T.
AU - Pereboev, Alexander
N1 - Funding Information:
We thank Drs. Joanne Douglas, Theresa Strong, Selvarangan Ponnazhagan, and Eric Hunter for their careful review of the manuscript. The work in this manuscript was supported by the National Institutes of Health Grants RO1 CA94084, RO1 CA83821, RO1 CA93796, and P50 CA83591, and the Komen Foundation.
PY - 2006/12
Y1 - 2006/12
N2 - Alternate serotypes of adenovirus (Ad), including Ads of species B, are being explored to circumvent the disadvantages of Ad serotype 5 gene delivery vectors. Whereas the majority of human Ads utilize the Coxsackievirus and adenovirus receptor (CAR), none of the Ad species B use CAR. Ad species B is further divided into two subspecies, B1 and B2, and utilizes at least two classes of receptors: common Ad species B receptors and B2 specific receptors. CD46 has been implicated as a B2-specific receptor. Ad serotype 3 (Ad3), a member of B1, utilizes CD80 and CD86 as cellular attachment receptors. The receptor-interacting Ad fiber-knob domain is highly homologous among species B Ads. We hypothesized that other members of Ad species B may utilize CD80 and CD86 as cellular attachment receptors. All tested species B members showed specific binding to cells expressing CD80 and CD86, and the Ad fiber-knob domain from both B1 and B2 Ad efficiently blocked CD80- and CD86-mediated infection of Ad3 vectors. Members of both B1 and B2 demonstrated CD80- and CD86-specific infection of CHO cells expressing CD80 and CD86. Therefore, all of the members of Ad species B utilize CD80 and CD86 for infection of cells.
AB - Alternate serotypes of adenovirus (Ad), including Ads of species B, are being explored to circumvent the disadvantages of Ad serotype 5 gene delivery vectors. Whereas the majority of human Ads utilize the Coxsackievirus and adenovirus receptor (CAR), none of the Ad species B use CAR. Ad species B is further divided into two subspecies, B1 and B2, and utilizes at least two classes of receptors: common Ad species B receptors and B2 specific receptors. CD46 has been implicated as a B2-specific receptor. Ad serotype 3 (Ad3), a member of B1, utilizes CD80 and CD86 as cellular attachment receptors. The receptor-interacting Ad fiber-knob domain is highly homologous among species B Ads. We hypothesized that other members of Ad species B may utilize CD80 and CD86 as cellular attachment receptors. All tested species B members showed specific binding to cells expressing CD80 and CD86, and the Ad fiber-knob domain from both B1 and B2 Ad efficiently blocked CD80- and CD86-mediated infection of Ad3 vectors. Members of both B1 and B2 demonstrated CD80- and CD86-specific infection of CHO cells expressing CD80 and CD86. Therefore, all of the members of Ad species B utilize CD80 and CD86 for infection of cells.
KW - Adenovirus species B
KW - CD80
KW - CD86
KW - Receptor
UR - http://www.scopus.com/inward/record.url?scp=33751017951&partnerID=8YFLogxK
U2 - 10.1016/j.virusres.2006.07.009
DO - 10.1016/j.virusres.2006.07.009
M3 - Article
C2 - 16920215
AN - SCOPUS:33751017951
SN - 0168-1702
VL - 122
SP - 144
EP - 153
JO - Virus Research
JF - Virus Research
IS - 1-2
ER -